Expression of linoleate isomerase gene from Lactobacillus reuteri PYR8 in Pichia pastoris

Linoleate isomerase gene was amplified by PCR from chromosome of Lactobacillus reuteri PYR8, then the gene was cloned into Pichia pastoris expression vector pPIC9K. The recombinant plasmid pPIC9K-LI was transformed into P. pastoris GS115 by electroporation and was induced with 1% methanol, it was de...

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Bibliographic Details

Published in:	浙江大学学报. 农业与生命科学版
Main Authors:	ZHANG Yan-he, ZHANG Lan-wei, HU Sen, BU Zhi-gao
Format:	Article
Language:	English
Published:	Zhejiang University Press 2006-09-01
Subjects:	<italic>Pichia pastoris</italic> <italic>Lactobacillus reuteri</italic> PYR8 linoleate isomerase CLA gas chromatography
Online Access:	https://www.academax.com/doi/10.3785/1008-9209.2006.05.0505