An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for <i>Salmonella enterica</i>: Potential for Integration into Poultry Feed
This research work aimed at developing an edible biopolymeric microcapsular wrapping (EBMW) integrating lytic bacteriophage particles for <i>Salmonella enterica</i>, with potential application in poultry feed for biocontrol of that pathogen. This pathogen is known as one of the main micr...
| 發表在: | Antibiotics |
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| Main Authors: | , , , , , , , |
| 格式: | Article |
| 語言: | 英语 |
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MDPI AG
2023-05-01
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| 在線閱讀: | https://www.mdpi.com/2079-6382/12/6/988 |
| _version_ | 1850402081182056448 |
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| author | Arthur O. Pereira Nicole M. A. Barros Bruna R. Guerrero Stephen C. Emencheta Denicezar Â. Baldo José M. Oliveira Jr. Marta M. D. C. Vila Victor M. Balcão |
| author_facet | Arthur O. Pereira Nicole M. A. Barros Bruna R. Guerrero Stephen C. Emencheta Denicezar Â. Baldo José M. Oliveira Jr. Marta M. D. C. Vila Victor M. Balcão |
| author_sort | Arthur O. Pereira |
| collection | DOAJ |
| container_title | Antibiotics |
| description | This research work aimed at developing an edible biopolymeric microcapsular wrapping (EBMW) integrating lytic bacteriophage particles for <i>Salmonella enterica</i>, with potential application in poultry feed for biocontrol of that pathogen. This pathogen is known as one of the main microorganisms responsible for contamination in the food industry and in foodstuff. The current techniques for decontamination and pathogen control in the food industry can be very expensive, not very selective, and even outdated, such as the use of broad-spectrum antibiotics that end up selecting resistant bacteria. Hence, there is a need for new technologies for pathogen biocontrol. In this context, bacteriophage-based biocontrol appears as a potential alternative. As a cocktail, both phages were able to significantly reduce the bacterial load after 12 h of treatment, at either multiplicity of infection (MOI) 1 and 10, by 84.3% and 87.6%, respectively. Entrapment of the phage virions within the EBMW matrix did not exert any deleterious effect upon their lytic activity. The results obtained showed high promise for integration in poultry feed aiming at controlling <i>Salmonella enterica</i>, since the edible biopolymeric microcapsular wrapping integrating lytic bacteriophage particles developed was successful in maintaining lytic phage viability while fully stabilizing the phage particles. |
| format | Article |
| id | doaj-art-ee5841ce2d7a4fb8b0bfefef9984149d |
| institution | Directory of Open Access Journals |
| issn | 2079-6382 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-ee5841ce2d7a4fb8b0bfefef9984149d2025-08-19T22:49:47ZengMDPI AGAntibiotics2079-63822023-05-0112698810.3390/antibiotics12060988An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for <i>Salmonella enterica</i>: Potential for Integration into Poultry FeedArthur O. Pereira0Nicole M. A. Barros1Bruna R. Guerrero2Stephen C. Emencheta3Denicezar Â. Baldo4José M. Oliveira Jr.5Marta M. D. C. Vila6Victor M. Balcão7PhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba 18023-000, SP, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba 18023-000, SP, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba 18023-000, SP, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba 18023-000, SP, BrazilLaFiNAU—Laboratory of Applied Nuclear Physics, University of Sorocaba, Sorocaba 18023-000, SP, BrazilLaFiNAU—Laboratory of Applied Nuclear Physics, University of Sorocaba, Sorocaba 18023-000, SP, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba 18023-000, SP, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba 18023-000, SP, BrazilThis research work aimed at developing an edible biopolymeric microcapsular wrapping (EBMW) integrating lytic bacteriophage particles for <i>Salmonella enterica</i>, with potential application in poultry feed for biocontrol of that pathogen. This pathogen is known as one of the main microorganisms responsible for contamination in the food industry and in foodstuff. The current techniques for decontamination and pathogen control in the food industry can be very expensive, not very selective, and even outdated, such as the use of broad-spectrum antibiotics that end up selecting resistant bacteria. Hence, there is a need for new technologies for pathogen biocontrol. In this context, bacteriophage-based biocontrol appears as a potential alternative. As a cocktail, both phages were able to significantly reduce the bacterial load after 12 h of treatment, at either multiplicity of infection (MOI) 1 and 10, by 84.3% and 87.6%, respectively. Entrapment of the phage virions within the EBMW matrix did not exert any deleterious effect upon their lytic activity. The results obtained showed high promise for integration in poultry feed aiming at controlling <i>Salmonella enterica</i>, since the edible biopolymeric microcapsular wrapping integrating lytic bacteriophage particles developed was successful in maintaining lytic phage viability while fully stabilizing the phage particles.https://www.mdpi.com/2079-6382/12/6/988bacteriophage particles<i>Salmonella enterica</i>edible biopolymeric wrappingfoodborne illnessphage cocktailantibacterial control |
| spellingShingle | Arthur O. Pereira Nicole M. A. Barros Bruna R. Guerrero Stephen C. Emencheta Denicezar Â. Baldo José M. Oliveira Jr. Marta M. D. C. Vila Victor M. Balcão An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for <i>Salmonella enterica</i>: Potential for Integration into Poultry Feed bacteriophage particles <i>Salmonella enterica</i> edible biopolymeric wrapping foodborne illness phage cocktail antibacterial control |
| title | An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for <i>Salmonella enterica</i>: Potential for Integration into Poultry Feed |
| title_full | An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for <i>Salmonella enterica</i>: Potential for Integration into Poultry Feed |
| title_fullStr | An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for <i>Salmonella enterica</i>: Potential for Integration into Poultry Feed |
| title_full_unstemmed | An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for <i>Salmonella enterica</i>: Potential for Integration into Poultry Feed |
| title_short | An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for <i>Salmonella enterica</i>: Potential for Integration into Poultry Feed |
| title_sort | edible biopolymeric microcapsular wrapping integrating lytic bacteriophage particles for i salmonella enterica i potential for integration into poultry feed |
| topic | bacteriophage particles <i>Salmonella enterica</i> edible biopolymeric wrapping foodborne illness phage cocktail antibacterial control |
| url | https://www.mdpi.com/2079-6382/12/6/988 |
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