New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe

We describe a series of new vectors for PCR-based epitope tagging and gene disruption in the fission yeast Schizosaccharomyces pombe, an exceptional model organism for the study of cellular processes. The vectors are designed for amplification of gene-targeting DNA cassettes and integration into spe...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	Mariana C. Gadaleta, Osamu Iwasaki, Chiaki Noguchi, Ken-ichi Noma, Eishi Noguchi
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2013-11-01
Subjects:	Schizosaccharomyces pombe Pk epitope tagging FLAG tagging glycine linker fluorescent tagging gene disruption
Online Access:	https://www.future-science.com/doi/10.2144/000114100