Optimising high-throughput sequencing data analysis, from gene database selection to the analysis of compositional data: a case study on tropical soil nematodes

IntroductionHigh-throughput sequencing (HTS) provides an efficient and cost-effective way to generate large amounts of sequence data, providing a very powerful tool to analyze biodiversity of soil organisms. However, marker-based methods and the resulting datasets come with a range of challenges and...

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Bibliographic Details

Published in:	Frontiers in Ecology and Evolution
Main Authors:	Simin Wang, Dominik Schneider, Tamara R. Hartke, Johannes Ballauff, Carina Carneiro de Melo Moura, Garvin Schulz, Zhipeng Li, Andrea Polle, Rolf Daniel, Oliver Gailing, Bambang Irawan, Stefan Scheu, Valentyna Krashevska
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-03-01
Subjects:	18S rRNA gene reference database amplicon sequence variant (ASV) operational taxonomic unit (OTU) data normalization method
Online Access:	https://www.frontiersin.org/articles/10.3389/fevo.2024.1168288/full