Protocol for generation of multicellular spheroids through reduced gravity

Summary: Multicellular spheroids are useful models for drug testing or studying tumor biology, but their production requires specialized approaches. Here, we present a protocol to produce viable spheroids by slow rotation around a horizontal axis using standard culture tubes. We describe steps for b...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Dylan A. Zinn, Christine Mehner, Tushar Patel
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	Cell Biology Cell culture Cell-based Assays Cancer Organoids
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723002228