Fine-tuning of a CRISPRi screen in the seventh pandemic Vibrio cholerae

Abstract Background Vibrio cholerae O1 El Tor, the etiological agent responsible for the last cholera pandemic, has become a well-established model organism for which some genetic tools are available. While CRISPRi technology has been applied to V. cholerae, improvements were necessary to upscale it...

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Bibliographic Details
Published in:BMC Genomics
Main Authors: Kevin Debatisse, Théophile Niault, Sarah Peeters, Amandine Maire, Busra Toktas, Baptiste Darracq, Zeynep Baharoglu, David Bikard, Didier Mazel, Céline Loot
Format: Article
Language:English
Published: BMC 2024-10-01
Subjects:
CRISPRi
Vibrio cholerae
Pooled screening
Essential genes
sgRNA library
Online Access:https://doi.org/10.1186/s12864-024-10891-1

Internet

https://doi.org/10.1186/s12864-024-10891-1

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