Cultivation of previously uncultured sponge-associated bacteria using advanced cultivation techniques: A perspective on possible key mechanisms

Most of the microbes from natural habitats cannot be cultivated with standard cultivation in laboratory, and sponge-associated microbes are no exception. We used two advanced methods based on a continuous-flow bioreactor (CF) and in situ cultivation (I-tip) to isolate previously uncultivated marine...

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Bibliographic Details

Published in:	Frontiers in Marine Science
Main Authors:	Dawoon Jung, Koshi Machida, Yoichi Nakao, Jeffrey S. Owen, Shan He, Tomonori Kindaichi, Akiyoshi Ohashi, Yoshiteru Aoi
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2022-08-01
Subjects:	uncultured microbes cultivation continuous-flow bioreactor slow-growing bacteria in situ cultivation I-tip cultivation
Online Access:	https://www.frontiersin.org/articles/10.3389/fmars.2022.963277/full