Optimizing thyroxine levels for enhanced buffalo sperm cryopreservation and fertility: a focus on quality, viability, and antioxidant protection

IntroductionThis study investigated the effects of adding thyroxine (T4) to buffalo semen on sperm quality, oxidative markers, apoptosis-like changes, and fertility.MethodsInitially, we tested a wide range of T4 concentrations (0.1, 0.3, 0.9, 2.7, and 8.1 µg/dL) to evaluate their impact on motility...

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Bibliographic Details

Published in:	Frontiers in Veterinary Science
Main Authors:	Maha S. Salama, Mohey A. Ashour, Ahmed M. Shehabeldin, Mohamed E. A. Omar, Mohamed M. Soliman, Heba I. Ghamry, Mohamed Abdelmegeid, Mustafa Shukry, Ahmed A. Elolimy
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-04-01
Subjects:	thyroxine (T4) sperm cryopreservation apoptosis-like changes fertility rate buffalo bull
Online Access:	https://www.frontiersin.org/articles/10.3389/fvets.2025.1584903/full