Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells

Abstract CRISPR/Cas9-mediated gene editing has great potential utility for treating genetic diseases. However, its therapeutic applications are limited by unintended genomic alterations arising from DNA double-strand breaks and random integration of exogenous DNA. In this study, we propose NICER, a...

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Bibliographic Details

Published in:	Nature Communications
Main Authors:	Akiko Tomita, Hiroyuki Sasanuma, Tomoo Owa, Yuka Nakazawa, Mayuko Shimada, Takahiro Fukuoka, Tomoo Ogi, Shinichiro Nakada
Format:	Article
Language:	English
Published:	Nature Portfolio 2023-09-01
Online Access:	https://doi.org/10.1038/s41467-023-41048-5