An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation

Abstract Objective Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the dynamic behavior of plant genes and the...

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Bibliographic Details

Published in:	BMC Research Notes
Main Authors:	Jeffrey R. Allen, Michael Flanagan, Sunita Pathak, Samantha K. Emenecker, Ryan J. Emenecker, Lucia C. Strader
Format:	Article
Language:	English
Published:	BMC 2025-04-01
Subjects:	Protoplast transfection Agrobacterium transformation Transgenic plant Plant transformation Transient expression Live cell imaging
Online Access:	https://doi.org/10.1186/s13104-025-07231-1