Three-dimensional single-cell transcriptome imaging of thick tissues

Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows genome-scale imaging of RNAs in individual cells in intact tissues. To date, MERFISH has been applied to image thin-tissue samples of ~10 µm thickness. Here, we present a thick-tissue three-dimensional (3D) MERFISH imaging...

وصف كامل

التفاصيل البيبلوغرافية

الحاوية / القاعدة:	eLife
المؤلفون الرئيسيون:	Rongxin Fang, Aaron Halpern, Mohammed Mostafizur Rahman, Zhengkai Huang, Zhiyun Lei, Sebastian J Hell, Catherine Dulac, Xiaowei Zhuang
التنسيق:	مقال
اللغة:	الإنجليزية
منشور في:	eLife Sciences Publications Ltd 2024-12-01
الموضوعات:	spatial genomics MERFISH brain genome-scale imaging thick-tissue imaging spatial transcriptomics
الوصول للمادة أونلاين:	https://elifesciences.org/articles/90029