Three-dimensional single-cell transcriptome imaging of thick tissues

Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows genome-scale imaging of RNAs in individual cells in intact tissues. To date, MERFISH has been applied to image thin-tissue samples of ~10 µm thickness. Here, we present a thick-tissue three-dimensional (3D) MERFISH imaging...

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Bibliographic Details
Published in:	eLife
Main Authors:	Rongxin Fang, Aaron Halpern, Mohammed Mostafizur Rahman, Zhengkai Huang, Zhiyun Lei, Sebastian J Hell, Catherine Dulac, Xiaowei Zhuang
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2024-12-01
Subjects:	spatial genomics MERFISH brain genome-scale imaging thick-tissue imaging spatial transcriptomics
Online Access:	https://elifesciences.org/articles/90029

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