A Pilot Study to Non-Invasively Track PIK3CA Mutation in Head and Neck Cancer
Background: PIK3CA pathways are the most frequently mutated oncogenic pathway in head and neck squamous cell carcinoma (HNSCC), including virally driven HNCs. PIK3CA is involved in the PI3K-PTEN-mTOR signalling pathway. PIK3CA has been implicated in HNSCC progression and PIK3CA mutations may serve a...
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doaj-0006cf3c426d4007b6008fdbe4c042af2020-11-25T00:37:30ZengMDPI AGDiagnostics2075-44182018-11-01847910.3390/diagnostics8040079diagnostics8040079A Pilot Study to Non-Invasively Track PIK3CA Mutation in Head and Neck CancerHenri Schmidt0Arutha Kulasinghe1Richard J.N. Allcock2Lit Yeen Tan3Elisa Mokany4Liz Kenny5Chamindie Punyadeera6The School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove 4059, Queensland, AustraliaThe School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove 4059, Queensland, AustraliaSchool of Biomedical sciences, The University of Western Australia, Nedlands 6009, Western Australia, AustraliaSpeeDx Pty. Ltd., National Innovation Centre, Australian Technology Park, Eveleigh Sydney 2015, New South Wales, AustraliaSpeeDx Pty. Ltd., National Innovation Centre, Australian Technology Park, Eveleigh Sydney 2015, New South Wales, AustraliaCentral Integrated Regional Cancer Service, Royal Brisbane and Women’s Hospital, Herston 4029, Queensland, AustraliaThe School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove 4059, Queensland, AustraliaBackground: PIK3CA pathways are the most frequently mutated oncogenic pathway in head and neck squamous cell carcinoma (HNSCC), including virally driven HNCs. PIK3CA is involved in the PI3K-PTEN-mTOR signalling pathway. PIK3CA has been implicated in HNSCC progression and PIK3CA mutations may serve as predictive biomarkers for therapy selection. Circulating tumour DNA (ctDNA) derived from necrotic and apoptotic tumour cells are thought to harbour tumour-specific genetic alterations. As such, the detection of PIK3CA alterations detected by ctDNA holds promise as a potential biomarker in HNSCC. Methods: Blood samples from treatment naïve HNSCC patients (<i>n</i> = 29) were interrogated for a commonly mutated PIK3CA hotspot mutation using low cost allele-specific Plex-PCR<sup>TM</sup> technology. Results: In this pilot, cross sectional study, PIK3CA E545K mutation was detected in the plasma samples of 9/29 HNSCC patients using the Plex-PCR<sup>TM</sup> technology. Conclusion: The results of this pilot study support the notion of using allele-specific technologies for cost-effective testing of ctDNA, and further assert the potential utility of ctDNA in HNSCC.https://www.mdpi.com/2075-4418/8/4/79circulating tumour (ctDNA), liquid biopsieshead and neck squamous cell carcinomahead and neck cancersdiagnosismonitoring |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Henri Schmidt Arutha Kulasinghe Richard J.N. Allcock Lit Yeen Tan Elisa Mokany Liz Kenny Chamindie Punyadeera |
spellingShingle |
Henri Schmidt Arutha Kulasinghe Richard J.N. Allcock Lit Yeen Tan Elisa Mokany Liz Kenny Chamindie Punyadeera A Pilot Study to Non-Invasively Track PIK3CA Mutation in Head and Neck Cancer Diagnostics circulating tumour (ctDNA), liquid biopsies head and neck squamous cell carcinoma head and neck cancers diagnosis monitoring |
author_facet |
Henri Schmidt Arutha Kulasinghe Richard J.N. Allcock Lit Yeen Tan Elisa Mokany Liz Kenny Chamindie Punyadeera |
author_sort |
Henri Schmidt |
title |
A Pilot Study to Non-Invasively Track PIK3CA Mutation in Head and Neck Cancer |
title_short |
A Pilot Study to Non-Invasively Track PIK3CA Mutation in Head and Neck Cancer |
title_full |
A Pilot Study to Non-Invasively Track PIK3CA Mutation in Head and Neck Cancer |
title_fullStr |
A Pilot Study to Non-Invasively Track PIK3CA Mutation in Head and Neck Cancer |
title_full_unstemmed |
A Pilot Study to Non-Invasively Track PIK3CA Mutation in Head and Neck Cancer |
title_sort |
pilot study to non-invasively track pik3ca mutation in head and neck cancer |
publisher |
MDPI AG |
series |
Diagnostics |
issn |
2075-4418 |
publishDate |
2018-11-01 |
description |
Background: PIK3CA pathways are the most frequently mutated oncogenic pathway in head and neck squamous cell carcinoma (HNSCC), including virally driven HNCs. PIK3CA is involved in the PI3K-PTEN-mTOR signalling pathway. PIK3CA has been implicated in HNSCC progression and PIK3CA mutations may serve as predictive biomarkers for therapy selection. Circulating tumour DNA (ctDNA) derived from necrotic and apoptotic tumour cells are thought to harbour tumour-specific genetic alterations. As such, the detection of PIK3CA alterations detected by ctDNA holds promise as a potential biomarker in HNSCC. Methods: Blood samples from treatment naïve HNSCC patients (<i>n</i> = 29) were interrogated for a commonly mutated PIK3CA hotspot mutation using low cost allele-specific Plex-PCR<sup>TM</sup> technology. Results: In this pilot, cross sectional study, PIK3CA E545K mutation was detected in the plasma samples of 9/29 HNSCC patients using the Plex-PCR<sup>TM</sup> technology. Conclusion: The results of this pilot study support the notion of using allele-specific technologies for cost-effective testing of ctDNA, and further assert the potential utility of ctDNA in HNSCC. |
topic |
circulating tumour (ctDNA), liquid biopsies head and neck squamous cell carcinoma head and neck cancers diagnosis monitoring |
url |
https://www.mdpi.com/2075-4418/8/4/79 |
work_keys_str_mv |
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