The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration
Capsid-like particle (CLP) displays can be used to enhance the immunogenicity of vaccine antigens, but a better understanding of how CLP vaccines are best formulated and delivered is needed. This study compared the humoral immune responses in mice elicited against two different vaccine antigens (a b...
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doaj-0008c113a9bf495698d73e1a4d92b23b2021-02-07T00:03:07ZengMDPI AGVaccines2076-393X2021-02-01913113110.3390/vaccines9020131The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of AdministrationChristoph M. Janitzek0Philip H. R. Carlsen1Susan Thrane2Vijansh M. Khanna3Virginie Jakob4Christophe Barnier-Quer5Nicolas Collin6Thor G. Theander7Ali Salanti8Morten A. Nielsen9Adam F. Sander10Centre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, 1165 København, DenmarkCentre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, 1165 København, DenmarkCentre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, 1165 København, DenmarkCentre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, 1165 København, DenmarkVaccine Formulation Institute, Plan-Les-Ouates, 1228 Geneva, SwitzerlandVaccine Formulation Laboratory, University of Lausanne, 1015 Lausanne, SwitzerlandVaccine Formulation Laboratory, University of Lausanne, 1015 Lausanne, SwitzerlandCentre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, 1165 København, DenmarkCentre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, 1165 København, DenmarkCentre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, 1165 København, DenmarkCentre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, 1165 København, DenmarkCapsid-like particle (CLP) displays can be used to enhance the immunogenicity of vaccine antigens, but a better understanding of how CLP vaccines are best formulated and delivered is needed. This study compared the humoral immune responses in mice elicited against two different vaccine antigens (a bacterial protein and a viral peptide) delivered on an AP205 CLP platform using six different adjuvant formulations. In comparison to antibody responses obtained after immunization with the unadjuvanted CLP vaccine, three of the adjuvant systems (neutral liposomes/monophosphoryl lipid A/quillaja saponaria 21, squalene-in-water emulsion, and monophosphoryl lipid A) caused significantly increased antibody levels, whereas formulation with the three other adjuvants (aluminum hydroxide, cationic liposomes, and cationic microparticles) resulted in similar or even decreased antibody responses. When delivering the soluble bacterial protein in a squalene-in-water emulsion, 4-log lower IgG levels were obtained compared to when the protein was delivered on CLPs without the adjuvant. The AP205 CLP platform promoted induction of both IgG1 and IgG2 subclasses, which could be skewed towards a higher production of IgG1 (aluminum hydroxide). Compared to other routes, intramuscular administration elicited the highest IgG levels. These results indicate that the effect of the external adjuvant does not always synergize with the adjuvant effect of the CLP display, which underscores the need for empirical testing of different extrinsic adjuvants.https://www.mdpi.com/2076-393X/9/2/131vaccinecapsid-like particlevirus-like particleadjuvantsAP205route of immunization |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christoph M. Janitzek Philip H. R. Carlsen Susan Thrane Vijansh M. Khanna Virginie Jakob Christophe Barnier-Quer Nicolas Collin Thor G. Theander Ali Salanti Morten A. Nielsen Adam F. Sander |
spellingShingle |
Christoph M. Janitzek Philip H. R. Carlsen Susan Thrane Vijansh M. Khanna Virginie Jakob Christophe Barnier-Quer Nicolas Collin Thor G. Theander Ali Salanti Morten A. Nielsen Adam F. Sander The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration Vaccines vaccine capsid-like particle virus-like particle adjuvants AP205 route of immunization |
author_facet |
Christoph M. Janitzek Philip H. R. Carlsen Susan Thrane Vijansh M. Khanna Virginie Jakob Christophe Barnier-Quer Nicolas Collin Thor G. Theander Ali Salanti Morten A. Nielsen Adam F. Sander |
author_sort |
Christoph M. Janitzek |
title |
The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration |
title_short |
The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration |
title_full |
The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration |
title_fullStr |
The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration |
title_full_unstemmed |
The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration |
title_sort |
immunogenicity of capsid-like particle vaccines in combination with different adjuvants using different routes of administration |
publisher |
MDPI AG |
series |
Vaccines |
issn |
2076-393X |
publishDate |
2021-02-01 |
description |
Capsid-like particle (CLP) displays can be used to enhance the immunogenicity of vaccine antigens, but a better understanding of how CLP vaccines are best formulated and delivered is needed. This study compared the humoral immune responses in mice elicited against two different vaccine antigens (a bacterial protein and a viral peptide) delivered on an AP205 CLP platform using six different adjuvant formulations. In comparison to antibody responses obtained after immunization with the unadjuvanted CLP vaccine, three of the adjuvant systems (neutral liposomes/monophosphoryl lipid A/quillaja saponaria 21, squalene-in-water emulsion, and monophosphoryl lipid A) caused significantly increased antibody levels, whereas formulation with the three other adjuvants (aluminum hydroxide, cationic liposomes, and cationic microparticles) resulted in similar or even decreased antibody responses. When delivering the soluble bacterial protein in a squalene-in-water emulsion, 4-log lower IgG levels were obtained compared to when the protein was delivered on CLPs without the adjuvant. The AP205 CLP platform promoted induction of both IgG1 and IgG2 subclasses, which could be skewed towards a higher production of IgG1 (aluminum hydroxide). Compared to other routes, intramuscular administration elicited the highest IgG levels. These results indicate that the effect of the external adjuvant does not always synergize with the adjuvant effect of the CLP display, which underscores the need for empirical testing of different extrinsic adjuvants. |
topic |
vaccine capsid-like particle virus-like particle adjuvants AP205 route of immunization |
url |
https://www.mdpi.com/2076-393X/9/2/131 |
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