Selenium Nanoparticle Synthesized by <i>Proteus mirabilis</i> YC801: An Efficacious Pathway for Selenite Biotransformation and Detoxification
Selenite is extremely biotoxic, and as a result of this, exploitation of microorganisms able to reduce selenite to non-toxic elemental selenium (Se<sup>0</sup>) has attracted great interest. In this study, a bacterial strain exhibiting extreme tolerance to selenite (up to 100 mM) was iso...
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doaj-000c2787c0c34e4a8fafdb1f80eb22a12020-11-25T00:05:31ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-11-011912380910.3390/ijms19123809ijms19123809Selenium Nanoparticle Synthesized by <i>Proteus mirabilis</i> YC801: An Efficacious Pathway for Selenite Biotransformation and DetoxificationYuting Wang0Xian Shu1Jinyan Hou2Weili Lu3Weiwei Zhao4Shengwei Huang5Lifang Wu6Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaKey Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaKey Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaKey Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaKey Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaKey Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaKey Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaSelenite is extremely biotoxic, and as a result of this, exploitation of microorganisms able to reduce selenite to non-toxic elemental selenium (Se<sup>0</sup>) has attracted great interest. In this study, a bacterial strain exhibiting extreme tolerance to selenite (up to 100 mM) was isolated from the gut of adult <i>Monochamus alternatus</i> and identified as <i>Proteus mirabilis</i> YC801. This strain demonstrated efficient transformation of selenite into red selenium nanoparticles (SeNPs) by reducing nearly 100% of 1.0 and 5.0 mM selenite within 42 and 48 h, respectively. Electron microscopy and energy dispersive X-ray analysis demonstrated that the SeNPs were spherical and primarily localized extracellularly, with an average hydrodynamic diameter of 178.3 ± 11.5 nm. In vitro selenite reduction activity assays and real-time PCR indicated that thioredoxin reductase and similar proteins present in the cytoplasm were likely to be involved in selenite reduction, and that NADPH or NADH served as electron donors. Finally, Fourier-transform infrared spectral analysis confirmed the presence of protein and lipid residues on the surfaces of SeNPs. This is the first report on the capability of <i>P. mirabilis</i> to reduce selenite to SeNPs. <i>P. mirabilis</i> YC801 might provide an eco-friendly approach to bioremediate selenium-contaminated soil/water, as well as a bacterial catalyst for the biogenesis of SeNPs.https://www.mdpi.com/1422-0067/19/12/3809biogenic selenium nanoparticlesselenite reduction<i>Proteus mirabilis</i> YC801electron microscopy analysisFourier Transform Infrared (FTIR) SpectroscopyReal-time PCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuting Wang Xian Shu Jinyan Hou Weili Lu Weiwei Zhao Shengwei Huang Lifang Wu |
spellingShingle |
Yuting Wang Xian Shu Jinyan Hou Weili Lu Weiwei Zhao Shengwei Huang Lifang Wu Selenium Nanoparticle Synthesized by <i>Proteus mirabilis</i> YC801: An Efficacious Pathway for Selenite Biotransformation and Detoxification International Journal of Molecular Sciences biogenic selenium nanoparticles selenite reduction <i>Proteus mirabilis</i> YC801 electron microscopy analysis Fourier Transform Infrared (FTIR) Spectroscopy Real-time PCR |
author_facet |
Yuting Wang Xian Shu Jinyan Hou Weili Lu Weiwei Zhao Shengwei Huang Lifang Wu |
author_sort |
Yuting Wang |
title |
Selenium Nanoparticle Synthesized by <i>Proteus mirabilis</i> YC801: An Efficacious Pathway for Selenite Biotransformation and Detoxification |
title_short |
Selenium Nanoparticle Synthesized by <i>Proteus mirabilis</i> YC801: An Efficacious Pathway for Selenite Biotransformation and Detoxification |
title_full |
Selenium Nanoparticle Synthesized by <i>Proteus mirabilis</i> YC801: An Efficacious Pathway for Selenite Biotransformation and Detoxification |
title_fullStr |
Selenium Nanoparticle Synthesized by <i>Proteus mirabilis</i> YC801: An Efficacious Pathway for Selenite Biotransformation and Detoxification |
title_full_unstemmed |
Selenium Nanoparticle Synthesized by <i>Proteus mirabilis</i> YC801: An Efficacious Pathway for Selenite Biotransformation and Detoxification |
title_sort |
selenium nanoparticle synthesized by <i>proteus mirabilis</i> yc801: an efficacious pathway for selenite biotransformation and detoxification |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-11-01 |
description |
Selenite is extremely biotoxic, and as a result of this, exploitation of microorganisms able to reduce selenite to non-toxic elemental selenium (Se<sup>0</sup>) has attracted great interest. In this study, a bacterial strain exhibiting extreme tolerance to selenite (up to 100 mM) was isolated from the gut of adult <i>Monochamus alternatus</i> and identified as <i>Proteus mirabilis</i> YC801. This strain demonstrated efficient transformation of selenite into red selenium nanoparticles (SeNPs) by reducing nearly 100% of 1.0 and 5.0 mM selenite within 42 and 48 h, respectively. Electron microscopy and energy dispersive X-ray analysis demonstrated that the SeNPs were spherical and primarily localized extracellularly, with an average hydrodynamic diameter of 178.3 ± 11.5 nm. In vitro selenite reduction activity assays and real-time PCR indicated that thioredoxin reductase and similar proteins present in the cytoplasm were likely to be involved in selenite reduction, and that NADPH or NADH served as electron donors. Finally, Fourier-transform infrared spectral analysis confirmed the presence of protein and lipid residues on the surfaces of SeNPs. This is the first report on the capability of <i>P. mirabilis</i> to reduce selenite to SeNPs. <i>P. mirabilis</i> YC801 might provide an eco-friendly approach to bioremediate selenium-contaminated soil/water, as well as a bacterial catalyst for the biogenesis of SeNPs. |
topic |
biogenic selenium nanoparticles selenite reduction <i>Proteus mirabilis</i> YC801 electron microscopy analysis Fourier Transform Infrared (FTIR) Spectroscopy Real-time PCR |
url |
https://www.mdpi.com/1422-0067/19/12/3809 |
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