Surface Design of Antifouling Vascular Constructs Bearing Biofunctional Peptides for Tissue Regeneration Applications
Antifouling polymer layers containing extracellular matrix-derived peptide motifs offer promising new options for biomimetic surface engineering. In this contribution, we report the design of antifouling vascular grafts bearing biofunctional peptide motifs for tissue regeneration applications based...
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doaj-00114ff3d644452eb42a634dbcfb9dfd2020-11-25T03:26:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-01216800680010.3390/ijms21186800Surface Design of Antifouling Vascular Constructs Bearing Biofunctional Peptides for Tissue Regeneration ApplicationsRadoslava Sivkova0Johanka Táborská1Alain Reparaz2Andres de los Santos Pereira3Ilya Kotelnikov4Vladimir Proks5Jan Kučka6Jan Svoboda7Tomáš Riedel8Ognen Pop-Georgievski9Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky sq. 2, 162 06 Prague, Czech RepublicAntifouling polymer layers containing extracellular matrix-derived peptide motifs offer promising new options for biomimetic surface engineering. In this contribution, we report the design of antifouling vascular grafts bearing biofunctional peptide motifs for tissue regeneration applications based on hierarchical polymer brushes. Hierarchical diblock poly(methyl ether oligo(ethylene glycol) methacrylate-<i>block</i>-glycidyl methacrylate) brushes bearing azide groups (poly(MeOEGMA-<i>block</i>-GMA-N<sub>3</sub>)) were grown by surface-initiated atom transfer radical polymerization (SI-ATRP) and functionalized with biomimetic RGD peptide sequences. Varying the conditions of copper-catalyzed alkyne-azide “click” reaction allowed for the immobilization of RGD peptides in a wide surface concentration range. The synthesized hierarchical polymer brushes bearing peptide motifs were characterized in detail using various surface sensitive physicochemical methods. The hierarchical brushes presenting the RGD sequences provided excellent cell adhesion properties and at the same time remained resistant to fouling from blood plasma. The synthesis of anti-fouling hierarchical brushes bearing 1.2 × 10<sup>3</sup> nmol/cm<sup>2 </sup>RGD biomimetic sequences has been adapted for the surface modification of commercially available grafts of woven polyethylene terephthalate (PET) fibers. The fiber mesh was endowed with polymerization initiator groups via aminolysis and acylation reactions optimized for the material. The obtained bioactive antifouling vascular grafts promoted the specific adhesion and growth of endothelial cells, thus providing a potential avenue for endothelialization of artificial conduits.https://www.mdpi.com/1422-0067/21/18/6800biomimetic surfacehierarchical bioactive polymer brushesvascular graft“click”-chemistryRGD peptideX-ray phоtoelectron spectroscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Radoslava Sivkova Johanka Táborská Alain Reparaz Andres de los Santos Pereira Ilya Kotelnikov Vladimir Proks Jan Kučka Jan Svoboda Tomáš Riedel Ognen Pop-Georgievski |
spellingShingle |
Radoslava Sivkova Johanka Táborská Alain Reparaz Andres de los Santos Pereira Ilya Kotelnikov Vladimir Proks Jan Kučka Jan Svoboda Tomáš Riedel Ognen Pop-Georgievski Surface Design of Antifouling Vascular Constructs Bearing Biofunctional Peptides for Tissue Regeneration Applications International Journal of Molecular Sciences biomimetic surface hierarchical bioactive polymer brushes vascular graft “click”-chemistry RGD peptide X-ray phоtoelectron spectroscopy |
author_facet |
Radoslava Sivkova Johanka Táborská Alain Reparaz Andres de los Santos Pereira Ilya Kotelnikov Vladimir Proks Jan Kučka Jan Svoboda Tomáš Riedel Ognen Pop-Georgievski |
author_sort |
Radoslava Sivkova |
title |
Surface Design of Antifouling Vascular Constructs Bearing Biofunctional Peptides for Tissue Regeneration Applications |
title_short |
Surface Design of Antifouling Vascular Constructs Bearing Biofunctional Peptides for Tissue Regeneration Applications |
title_full |
Surface Design of Antifouling Vascular Constructs Bearing Biofunctional Peptides for Tissue Regeneration Applications |
title_fullStr |
Surface Design of Antifouling Vascular Constructs Bearing Biofunctional Peptides for Tissue Regeneration Applications |
title_full_unstemmed |
Surface Design of Antifouling Vascular Constructs Bearing Biofunctional Peptides for Tissue Regeneration Applications |
title_sort |
surface design of antifouling vascular constructs bearing biofunctional peptides for tissue regeneration applications |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-09-01 |
description |
Antifouling polymer layers containing extracellular matrix-derived peptide motifs offer promising new options for biomimetic surface engineering. In this contribution, we report the design of antifouling vascular grafts bearing biofunctional peptide motifs for tissue regeneration applications based on hierarchical polymer brushes. Hierarchical diblock poly(methyl ether oligo(ethylene glycol) methacrylate-<i>block</i>-glycidyl methacrylate) brushes bearing azide groups (poly(MeOEGMA-<i>block</i>-GMA-N<sub>3</sub>)) were grown by surface-initiated atom transfer radical polymerization (SI-ATRP) and functionalized with biomimetic RGD peptide sequences. Varying the conditions of copper-catalyzed alkyne-azide “click” reaction allowed for the immobilization of RGD peptides in a wide surface concentration range. The synthesized hierarchical polymer brushes bearing peptide motifs were characterized in detail using various surface sensitive physicochemical methods. The hierarchical brushes presenting the RGD sequences provided excellent cell adhesion properties and at the same time remained resistant to fouling from blood plasma. The synthesis of anti-fouling hierarchical brushes bearing 1.2 × 10<sup>3</sup> nmol/cm<sup>2 </sup>RGD biomimetic sequences has been adapted for the surface modification of commercially available grafts of woven polyethylene terephthalate (PET) fibers. The fiber mesh was endowed with polymerization initiator groups via aminolysis and acylation reactions optimized for the material. The obtained bioactive antifouling vascular grafts promoted the specific adhesion and growth of endothelial cells, thus providing a potential avenue for endothelialization of artificial conduits. |
topic |
biomimetic surface hierarchical bioactive polymer brushes vascular graft “click”-chemistry RGD peptide X-ray phоtoelectron spectroscopy |
url |
https://www.mdpi.com/1422-0067/21/18/6800 |
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