Metagenomic data of free cyanide and thiocyanate degrading bacterial communities
The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertake...
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doaj-001d3a1733424bfb9cbe103ffad3ebab2020-11-25T03:28:30ZengElsevierData in Brief2352-34092017-08-0113738741Metagenomic data of free cyanide and thiocyanate degrading bacterial communitiesLukhanyo Mekuto0Seteno K.O. Ntwampe1John B.N. Mudumbi2Enoch A. Akinpelu3Maxwell Mewa-Ngongang4Corresponding authors.; Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 8000 South AfricaCorresponding authors.; Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 8000 South AfricaBioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 8000 South AfricaBioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 8000 South AfricaBioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 8000 South AfricaThe data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction kits as per manufacturer׳s instruction and sequenced using the illumina® MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database. Keywords: Cyanide degrading organisms, Thiocyanate degrading organisms, Metagenomics, 16S rRNA genehttp://www.sciencedirect.com/science/article/pii/S2352340917302937 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lukhanyo Mekuto Seteno K.O. Ntwampe John B.N. Mudumbi Enoch A. Akinpelu Maxwell Mewa-Ngongang |
spellingShingle |
Lukhanyo Mekuto Seteno K.O. Ntwampe John B.N. Mudumbi Enoch A. Akinpelu Maxwell Mewa-Ngongang Metagenomic data of free cyanide and thiocyanate degrading bacterial communities Data in Brief |
author_facet |
Lukhanyo Mekuto Seteno K.O. Ntwampe John B.N. Mudumbi Enoch A. Akinpelu Maxwell Mewa-Ngongang |
author_sort |
Lukhanyo Mekuto |
title |
Metagenomic data of free cyanide and thiocyanate degrading bacterial communities |
title_short |
Metagenomic data of free cyanide and thiocyanate degrading bacterial communities |
title_full |
Metagenomic data of free cyanide and thiocyanate degrading bacterial communities |
title_fullStr |
Metagenomic data of free cyanide and thiocyanate degrading bacterial communities |
title_full_unstemmed |
Metagenomic data of free cyanide and thiocyanate degrading bacterial communities |
title_sort |
metagenomic data of free cyanide and thiocyanate degrading bacterial communities |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2017-08-01 |
description |
The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction kits as per manufacturer׳s instruction and sequenced using the illumina® MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database. Keywords: Cyanide degrading organisms, Thiocyanate degrading organisms, Metagenomics, 16S rRNA gene |
url |
http://www.sciencedirect.com/science/article/pii/S2352340917302937 |
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