CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication
Carbon catabolite repression 4 (CCR4) is a conserved mRNA deadenylase regulating posttranscriptional gene expression. However, regulation of CCR4 in virus infections is less understood. Here, we characterized a pro-viral role of CCR4 in replication of a plant cytorhabdovirus, Barley yellow striate m...
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doaj-0027be40e7844874a1c92c0a75f2d8a62021-05-05T20:56:36ZengeLife Sciences Publications LtdeLife2050-084X2020-03-01910.7554/eLife.53753CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replicationZhen-Jia Zhang0https://orcid.org/0000-0003-0473-2738Qiang Gao1Xiao-Dong Fang2Zhi-Hang Ding3Dong-Min Gao4Wen-Ya Xu5Qing Cao6Ji-Hui Qiao7Yi-Zhou Yang8Chenggui Han9Ying Wang10Xuefeng Yuan11Dawei Li12Xian-Bing Wang13https://orcid.org/0000-0003-3082-2462State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaCollege of Plant Protection, China Agricultural University, Beijing, ChinaCollege of Plant Protection, China Agricultural University, Beijing, ChinaDepartment of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Shandong Province Key Laboratory of Agricultural Microbiology, Tai'an, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaState Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, ChinaCarbon catabolite repression 4 (CCR4) is a conserved mRNA deadenylase regulating posttranscriptional gene expression. However, regulation of CCR4 in virus infections is less understood. Here, we characterized a pro-viral role of CCR4 in replication of a plant cytorhabdovirus, Barley yellow striate mosaic virus (BYSMV). The barley (Hordeum vulgare) CCR4 protein (HvCCR4) was identified to interact with the BYSMV phosphoprotein (P). The BYSMV P protein recruited HvCCR4 from processing bodies (PBs) into viroplasm-like bodies. Overexpression of HvCCR4 promoted BYSMV replication in plants. Conversely, knockdown of the small brown planthopper CCR4 inhibited viral accumulation in the insect vector. Biochemistry experiments revealed that HvCCR4 was recruited into N–RNA complexes by the BYSMV P protein and triggered turnover of N-bound cellular mRNAs, thereby releasing RNA-free N protein to bind viral genomic RNA for optimal viral replication. Our results demonstrate that the co-opted CCR4-mediated RNA decay facilitates cytorhabdovirus replication in plants and insects.https://elifesciences.org/articles/53753plant cytorhabdovirusphosphoproteinCCR4RNA decayvirus-plant interactionbarley |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhen-Jia Zhang Qiang Gao Xiao-Dong Fang Zhi-Hang Ding Dong-Min Gao Wen-Ya Xu Qing Cao Ji-Hui Qiao Yi-Zhou Yang Chenggui Han Ying Wang Xuefeng Yuan Dawei Li Xian-Bing Wang |
spellingShingle |
Zhen-Jia Zhang Qiang Gao Xiao-Dong Fang Zhi-Hang Ding Dong-Min Gao Wen-Ya Xu Qing Cao Ji-Hui Qiao Yi-Zhou Yang Chenggui Han Ying Wang Xuefeng Yuan Dawei Li Xian-Bing Wang CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication eLife plant cytorhabdovirus phosphoprotein CCR4 RNA decay virus-plant interaction barley |
author_facet |
Zhen-Jia Zhang Qiang Gao Xiao-Dong Fang Zhi-Hang Ding Dong-Min Gao Wen-Ya Xu Qing Cao Ji-Hui Qiao Yi-Zhou Yang Chenggui Han Ying Wang Xuefeng Yuan Dawei Li Xian-Bing Wang |
author_sort |
Zhen-Jia Zhang |
title |
CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication |
title_short |
CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication |
title_full |
CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication |
title_fullStr |
CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication |
title_full_unstemmed |
CCR4, a RNA decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication |
title_sort |
ccr4, a rna decay factor, is hijacked by a plant cytorhabdovirus phosphoprotein to facilitate virus replication |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-03-01 |
description |
Carbon catabolite repression 4 (CCR4) is a conserved mRNA deadenylase regulating posttranscriptional gene expression. However, regulation of CCR4 in virus infections is less understood. Here, we characterized a pro-viral role of CCR4 in replication of a plant cytorhabdovirus, Barley yellow striate mosaic virus (BYSMV). The barley (Hordeum vulgare) CCR4 protein (HvCCR4) was identified to interact with the BYSMV phosphoprotein (P). The BYSMV P protein recruited HvCCR4 from processing bodies (PBs) into viroplasm-like bodies. Overexpression of HvCCR4 promoted BYSMV replication in plants. Conversely, knockdown of the small brown planthopper CCR4 inhibited viral accumulation in the insect vector. Biochemistry experiments revealed that HvCCR4 was recruited into N–RNA complexes by the BYSMV P protein and triggered turnover of N-bound cellular mRNAs, thereby releasing RNA-free N protein to bind viral genomic RNA for optimal viral replication. Our results demonstrate that the co-opted CCR4-mediated RNA decay facilitates cytorhabdovirus replication in plants and insects. |
topic |
plant cytorhabdovirus phosphoprotein CCR4 RNA decay virus-plant interaction barley |
url |
https://elifesciences.org/articles/53753 |
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