MiR-1193 Inhibits the Malignancy of Cervical Cancer Cells by Targeting Claudin 7 (CLDN7)
Bin Zhang,* Yao Lin,* Qiufang Bao, Yantong Zheng, Lan Lan Department of Obstetrics and Gynecology, The First Affiliated Hospital of Fujian Medical University, Fujian 350005, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bin Zhang Email zhangbin_fj...
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doaj-0070af29f64245d3a1543cb2c52b8dd82020-11-25T03:32:05ZengDove Medical PressOncoTargets and Therapy1178-69302020-05-01Volume 134349435853865MiR-1193 Inhibits the Malignancy of Cervical Cancer Cells by Targeting Claudin 7 (CLDN7)Zhang BLin YBao QZheng YLan LBin Zhang,* Yao Lin,* Qiufang Bao, Yantong Zheng, Lan Lan Department of Obstetrics and Gynecology, The First Affiliated Hospital of Fujian Medical University, Fujian 350005, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bin Zhang Email zhangbin_fj787@126.comObjective: MicroRNAs (miRNAs) are highly involved in cancer development, including in cervical cancer (CC). In this study, we aimed to investigate the role and possible mechanism of a poorly studied miRNA, miR-1193, in CC progression.Materials and Methods: Expression of miR-1193 was determined in 60 pairs of cervical samples. The impacts of miR-1193 on CC cell proliferation, invasion and migration capacities were verified by CCK-8, transwell and wound healing assays, respectively. Then, bioinformatics prediction, luciferase reporter assay, qRT-PCR and Western blot were successively conducted to study the targeting of claudin 7 (CLDN7) by miR-1193. After CLDN7 was restored in miR-1193-overexpressed cells, the rescue effects were determined. Finally, CLDN7 expression was analyzed in cervical samples, and its expression correlation with miR-1193 was explored.Results: Compared with paired normal tissues, miR-1193 was sharply decreased in abnormal tissues (intraepithelial lesions and cancerous tissues). Especially, miR-1193 expression was gradually decreased in low-grade squamous intraepithelial lesions, high-grade squamous intraepithelial lesions and CC. Enforced expression of miR-1193 inhibited CC cell proliferation, invasion and migration. Mechanistically, we confirmed CLDN7 as a target of miR-1193, and restoration of CLDN7 robustly rescued the tumor suppressing effects of miR-1193 in CC cells. CLDN7 was upregulated in abnormal cervical tissues and its expression exhibited inverse correlation with that of miR-1193 in CC.Conclusion: Our results suggested that miR-1193 exerted tumor inhibitory roles in CC malignancy by directly targeting CLDN7.Keywords: cervical cancer, miR-1193, tumor suppressing, CLDN7https://www.dovepress.com/mir-1193-inhibits-the-malignancy-of-cervical-cancer-cells-by-targeting-peer-reviewed-article-OTTcervical cancermir-1193tumor suppressingcldn7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhang B Lin Y Bao Q Zheng Y Lan L |
spellingShingle |
Zhang B Lin Y Bao Q Zheng Y Lan L MiR-1193 Inhibits the Malignancy of Cervical Cancer Cells by Targeting Claudin 7 (CLDN7) OncoTargets and Therapy cervical cancer mir-1193 tumor suppressing cldn7 |
author_facet |
Zhang B Lin Y Bao Q Zheng Y Lan L |
author_sort |
Zhang B |
title |
MiR-1193 Inhibits the Malignancy of Cervical Cancer Cells by Targeting Claudin 7 (CLDN7) |
title_short |
MiR-1193 Inhibits the Malignancy of Cervical Cancer Cells by Targeting Claudin 7 (CLDN7) |
title_full |
MiR-1193 Inhibits the Malignancy of Cervical Cancer Cells by Targeting Claudin 7 (CLDN7) |
title_fullStr |
MiR-1193 Inhibits the Malignancy of Cervical Cancer Cells by Targeting Claudin 7 (CLDN7) |
title_full_unstemmed |
MiR-1193 Inhibits the Malignancy of Cervical Cancer Cells by Targeting Claudin 7 (CLDN7) |
title_sort |
mir-1193 inhibits the malignancy of cervical cancer cells by targeting claudin 7 (cldn7) |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2020-05-01 |
description |
Bin Zhang,* Yao Lin,* Qiufang Bao, Yantong Zheng, Lan Lan Department of Obstetrics and Gynecology, The First Affiliated Hospital of Fujian Medical University, Fujian 350005, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bin Zhang Email zhangbin_fj787@126.comObjective: MicroRNAs (miRNAs) are highly involved in cancer development, including in cervical cancer (CC). In this study, we aimed to investigate the role and possible mechanism of a poorly studied miRNA, miR-1193, in CC progression.Materials and Methods: Expression of miR-1193 was determined in 60 pairs of cervical samples. The impacts of miR-1193 on CC cell proliferation, invasion and migration capacities were verified by CCK-8, transwell and wound healing assays, respectively. Then, bioinformatics prediction, luciferase reporter assay, qRT-PCR and Western blot were successively conducted to study the targeting of claudin 7 (CLDN7) by miR-1193. After CLDN7 was restored in miR-1193-overexpressed cells, the rescue effects were determined. Finally, CLDN7 expression was analyzed in cervical samples, and its expression correlation with miR-1193 was explored.Results: Compared with paired normal tissues, miR-1193 was sharply decreased in abnormal tissues (intraepithelial lesions and cancerous tissues). Especially, miR-1193 expression was gradually decreased in low-grade squamous intraepithelial lesions, high-grade squamous intraepithelial lesions and CC. Enforced expression of miR-1193 inhibited CC cell proliferation, invasion and migration. Mechanistically, we confirmed CLDN7 as a target of miR-1193, and restoration of CLDN7 robustly rescued the tumor suppressing effects of miR-1193 in CC cells. CLDN7 was upregulated in abnormal cervical tissues and its expression exhibited inverse correlation with that of miR-1193 in CC.Conclusion: Our results suggested that miR-1193 exerted tumor inhibitory roles in CC malignancy by directly targeting CLDN7.Keywords: cervical cancer, miR-1193, tumor suppressing, CLDN7 |
topic |
cervical cancer mir-1193 tumor suppressing cldn7 |
url |
https://www.dovepress.com/mir-1193-inhibits-the-malignancy-of-cervical-cancer-cells-by-targeting-peer-reviewed-article-OTT |
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