A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation
<p>Abstract</p> <p>Background</p> <p>Neurons assemble into a functional network through a sequence of developmental processes including neuronal polarization and synapse formation. In <it>Caenorhabditis elegans</it>, the serine/threonine SAD-1 kinase is esse...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2008-09-01
|
Series: | Neural Development |
Online Access: | http://www.neuraldevelopment.com/content/3/1/23 |
id |
doaj-00787473809e44debad5cb4fed57c501 |
---|---|
record_format |
Article |
spelling |
doaj-00787473809e44debad5cb4fed57c5012020-11-24T23:31:47ZengBMCNeural Development1749-81042008-09-01312310.1186/1749-8104-3-23A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formationShokat Kevan MZhang ChaoLilley Brendan NKim Joanne SMSanes Joshua RZhen Mei<p>Abstract</p> <p>Background</p> <p>Neurons assemble into a functional network through a sequence of developmental processes including neuronal polarization and synapse formation. In <it>Caenorhabditis elegans</it>, the serine/threonine SAD-1 kinase is essential for proper neuronal polarity and synaptic organization. To determine if SAD-1 activity regulates the establishment or maintenance of these neuronal structures, we examined its temporal requirements using a chemical-genetic method that allows for selective and reversible inactivation of its kinase activity <it>in vivo</it>.</p> <p>Results</p> <p>We generated a PP1 analog-sensitive variant of SAD-1. Through temporal inhibition of SAD-1 kinase activity we show that its activity is required for the establishment of both neuronal polarity and synaptic organization. However, while SAD-1 activity is needed strictly when neurons are polarizing, the temporal requirement for SAD-1 is less stringent in synaptic organization, which can also be re-established during maintenance.</p> <p>Conclusion</p> <p>This study reports the first temporal analysis of a neural kinase activity using the chemical-genetic system. It reveals that neuronal polarity and synaptic organization have distinct temporal requirements for SAD-1.</p> http://www.neuraldevelopment.com/content/3/1/23 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shokat Kevan M Zhang Chao Lilley Brendan N Kim Joanne SM Sanes Joshua R Zhen Mei |
spellingShingle |
Shokat Kevan M Zhang Chao Lilley Brendan N Kim Joanne SM Sanes Joshua R Zhen Mei A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation Neural Development |
author_facet |
Shokat Kevan M Zhang Chao Lilley Brendan N Kim Joanne SM Sanes Joshua R Zhen Mei |
author_sort |
Shokat Kevan M |
title |
A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation |
title_short |
A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation |
title_full |
A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation |
title_fullStr |
A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation |
title_full_unstemmed |
A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation |
title_sort |
chemical-genetic strategy reveals distinct temporal requirements for sad-1 kinase in neuronal polarization and synapse formation |
publisher |
BMC |
series |
Neural Development |
issn |
1749-8104 |
publishDate |
2008-09-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Neurons assemble into a functional network through a sequence of developmental processes including neuronal polarization and synapse formation. In <it>Caenorhabditis elegans</it>, the serine/threonine SAD-1 kinase is essential for proper neuronal polarity and synaptic organization. To determine if SAD-1 activity regulates the establishment or maintenance of these neuronal structures, we examined its temporal requirements using a chemical-genetic method that allows for selective and reversible inactivation of its kinase activity <it>in vivo</it>.</p> <p>Results</p> <p>We generated a PP1 analog-sensitive variant of SAD-1. Through temporal inhibition of SAD-1 kinase activity we show that its activity is required for the establishment of both neuronal polarity and synaptic organization. However, while SAD-1 activity is needed strictly when neurons are polarizing, the temporal requirement for SAD-1 is less stringent in synaptic organization, which can also be re-established during maintenance.</p> <p>Conclusion</p> <p>This study reports the first temporal analysis of a neural kinase activity using the chemical-genetic system. It reveals that neuronal polarity and synaptic organization have distinct temporal requirements for SAD-1.</p> |
url |
http://www.neuraldevelopment.com/content/3/1/23 |
work_keys_str_mv |
AT shokatkevanm achemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT zhangchao achemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT lilleybrendann achemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT kimjoannesm achemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT sanesjoshuar achemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT zhenmei achemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT shokatkevanm chemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT zhangchao chemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT lilleybrendann chemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT kimjoannesm chemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT sanesjoshuar chemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation AT zhenmei chemicalgeneticstrategyrevealsdistincttemporalrequirementsforsad1kinaseinneuronalpolarizationandsynapseformation |
_version_ |
1725536092005859328 |