Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories
<p>Abstract</p> <p>Background</p> <p>The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing <it>Mycobacterium tuberculosis </it>(MTB) has been widely reported and it has an obvious clinical, therapeutic and social...
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doaj-00825933d9f94be5adfa9a37cbc495da2020-11-24T23:52:30ZengBMCBMC Microbiology1471-21802008-02-01813010.1186/1471-2180-8-30Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratoriesde Viedma DaríoBouza EmilioLirola MiguelFernández RosaHerranz MartaMartín Ana<p>Abstract</p> <p>Background</p> <p>The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing <it>Mycobacterium tuberculosis </it>(MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identification of the same MTB strain cultured from both the potential source of the contamination and from the false-positive candidate. The molecular tool usually applied in this context is IS6110-RFLP which takes a long time to provide an answer, usually longer than is acceptable for microbiologists and clinicians to make decisions. Our purpose in this study is to evaluate a novel PCR-based method, MIRU-VNTR as an alternative to assure a rapid and optimized analysis of cross-contamination alerts.</p> <p>Results</p> <p>MIRU-VNTR was prospectively compared with IS6110-RFLP for clarifying 19 alerts of false positivity from other laboratories. MIRU-VNTR highly correlated with IS6110-RFLP, reduced the response time by 27 days and clarified six alerts unresolved by RFLP. Additionally, MIRU-VNTR revealed complex situations such as contamination events involving polyclonal isolates and a false-positive case due to the simultaneous cross-contamination from two independent sources.</p> <p>Conclusion</p> <p>Unlike standard RFLP-based genotyping, MIRU-VNTR i) could help reduce the impact of a false positive diagnosis of TB, ii) increased the number of events that could be solved and iii) revealed the complexity of some cross-contamination events that could not be dissected by IS6110-RFLP.</p> http://www.biomedcentral.com/1471-2180/8/30 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
de Viedma Darío Bouza Emilio Lirola Miguel Fernández Rosa Herranz Marta Martín Ana |
spellingShingle |
de Viedma Darío Bouza Emilio Lirola Miguel Fernández Rosa Herranz Marta Martín Ana Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories BMC Microbiology |
author_facet |
de Viedma Darío Bouza Emilio Lirola Miguel Fernández Rosa Herranz Marta Martín Ana |
author_sort |
de Viedma Darío |
title |
Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories |
title_short |
Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories |
title_full |
Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories |
title_fullStr |
Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories |
title_full_unstemmed |
Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories |
title_sort |
optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2008-02-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing <it>Mycobacterium tuberculosis </it>(MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identification of the same MTB strain cultured from both the potential source of the contamination and from the false-positive candidate. The molecular tool usually applied in this context is IS6110-RFLP which takes a long time to provide an answer, usually longer than is acceptable for microbiologists and clinicians to make decisions. Our purpose in this study is to evaluate a novel PCR-based method, MIRU-VNTR as an alternative to assure a rapid and optimized analysis of cross-contamination alerts.</p> <p>Results</p> <p>MIRU-VNTR was prospectively compared with IS6110-RFLP for clarifying 19 alerts of false positivity from other laboratories. MIRU-VNTR highly correlated with IS6110-RFLP, reduced the response time by 27 days and clarified six alerts unresolved by RFLP. Additionally, MIRU-VNTR revealed complex situations such as contamination events involving polyclonal isolates and a false-positive case due to the simultaneous cross-contamination from two independent sources.</p> <p>Conclusion</p> <p>Unlike standard RFLP-based genotyping, MIRU-VNTR i) could help reduce the impact of a false positive diagnosis of TB, ii) increased the number of events that could be solved and iii) revealed the complexity of some cross-contamination events that could not be dissected by IS6110-RFLP.</p> |
url |
http://www.biomedcentral.com/1471-2180/8/30 |
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