Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs
Lipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of Leishmania infantum antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which a...
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doaj-008c8c4c3ebc4ac39dbb4b6f7f1add532021-03-12T04:58:01ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-03-01810.3389/fvets.2021.581148581148Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected DogsJulia Ramos Sampaio0Rodrigo Pedro Soares1Thiago Doria Barral2Gabriela Porfirio Passos3Maisa Santos Fonseca4Roberto Meyer5Stella Maria Barrouin-Melo6Ricardo Wagner Portela7Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilInstituto Rene Rachou, Fundação Oswaldo Cruz, Belo Horizonte, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilDepartamento de Anatomia, Patologia e Clínicas, Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, Salvador, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilDepartamento de Anatomia, Patologia e Clínicas, Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, Salvador, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilLipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of Leishmania infantum antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which allows their use in point-of-care testing without special storage conditions. During the glycoconjugate extraction, a glycoinositolphospholipid (GIPL)-enriched fraction is obtained in similar quantities as LPG. Since GIPLs can be extracted from the same parasite pellet as LPGs, this work aimed to evaluate the immune recognition of GIPLs by Leishmania infantum-infected dogs and its use for canine leishmaniasis (CanL) immunodiagnosis. Like LPG, GIPLs were recognized by sera from L. infantum-infected dogs, but with less sensitivity (83.8%). However, 80% (16/20) of subclinically infected dogs were detected as positive in the assay. Different from LPG, the GIPL-based assay achieved a lower specificity (73.7%) and cross-reactions occurred with T. cruzi and L. braziliensis-infected dogs. Although GIPLs exhibited a similar performance to LPG for subclinically L. infantum-infected dogs, the occurrence of cross-reactivities with other protozoa and a lower sensitivity hinders its use for an immunodiagnostic test. In places where those diseases do not co-exist such as in the Mediterranean region, its use for subclinically dogs could be an alternative.https://www.frontiersin.org/articles/10.3389/fvets.2021.581148/fullglycoconjugatesELISALeishmania infantumdogsimmunodiagnosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julia Ramos Sampaio Rodrigo Pedro Soares Thiago Doria Barral Gabriela Porfirio Passos Maisa Santos Fonseca Roberto Meyer Stella Maria Barrouin-Melo Ricardo Wagner Portela |
spellingShingle |
Julia Ramos Sampaio Rodrigo Pedro Soares Thiago Doria Barral Gabriela Porfirio Passos Maisa Santos Fonseca Roberto Meyer Stella Maria Barrouin-Melo Ricardo Wagner Portela Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs Frontiers in Veterinary Science glycoconjugates ELISA Leishmania infantum dogs immunodiagnosis |
author_facet |
Julia Ramos Sampaio Rodrigo Pedro Soares Thiago Doria Barral Gabriela Porfirio Passos Maisa Santos Fonseca Roberto Meyer Stella Maria Barrouin-Melo Ricardo Wagner Portela |
author_sort |
Julia Ramos Sampaio |
title |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
title_short |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
title_full |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
title_fullStr |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
title_full_unstemmed |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
title_sort |
leishmania infantum-derived glycoinositolphospholipids in the immunodiagnosis of subclinically infected dogs |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2021-03-01 |
description |
Lipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of Leishmania infantum antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which allows their use in point-of-care testing without special storage conditions. During the glycoconjugate extraction, a glycoinositolphospholipid (GIPL)-enriched fraction is obtained in similar quantities as LPG. Since GIPLs can be extracted from the same parasite pellet as LPGs, this work aimed to evaluate the immune recognition of GIPLs by Leishmania infantum-infected dogs and its use for canine leishmaniasis (CanL) immunodiagnosis. Like LPG, GIPLs were recognized by sera from L. infantum-infected dogs, but with less sensitivity (83.8%). However, 80% (16/20) of subclinically infected dogs were detected as positive in the assay. Different from LPG, the GIPL-based assay achieved a lower specificity (73.7%) and cross-reactions occurred with T. cruzi and L. braziliensis-infected dogs. Although GIPLs exhibited a similar performance to LPG for subclinically L. infantum-infected dogs, the occurrence of cross-reactivities with other protozoa and a lower sensitivity hinders its use for an immunodiagnostic test. In places where those diseases do not co-exist such as in the Mediterranean region, its use for subclinically dogs could be an alternative. |
topic |
glycoconjugates ELISA Leishmania infantum dogs immunodiagnosis |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2021.581148/full |
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