Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data
Eisenia nordenskioldi (Eisen, 1879) is the only autochthonous Siberian earthworm with a large distribution that ranges from tundra to steppe and broadleaved forests. This species has a very high morphological, ecological, karyological, and genetic diversity, so it was proposed that E. nordenskioldi...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2020.598196/full |
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| language |
English |
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Article |
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DOAJ |
| author |
Sergei V. Shekhovtsov Sergei V. Shekhovtsov Sergei V. Shekhovtsov Aleksandra A. Shipova Aleksandra A. Shipova Tatiana V. Poluboyarova Tatiana V. Poluboyarova Tatiana V. Poluboyarova Gennady V. Vasiliev Elena V. Golovanova Anna P. Geraskina Nina A. Bulakhova Nina A. Bulakhova Tímea Szederjesi Sergei E. Peltek Sergei E. Peltek |
| spellingShingle |
Sergei V. Shekhovtsov Sergei V. Shekhovtsov Sergei V. Shekhovtsov Aleksandra A. Shipova Aleksandra A. Shipova Tatiana V. Poluboyarova Tatiana V. Poluboyarova Tatiana V. Poluboyarova Gennady V. Vasiliev Elena V. Golovanova Anna P. Geraskina Nina A. Bulakhova Nina A. Bulakhova Tímea Szederjesi Sergei E. Peltek Sergei E. Peltek Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data Frontiers in Genetics phylogenomics phylogeny transcriptomes Lumbricidae Eisenia nordenskioldi earthworms |
| author_facet |
Sergei V. Shekhovtsov Sergei V. Shekhovtsov Sergei V. Shekhovtsov Aleksandra A. Shipova Aleksandra A. Shipova Tatiana V. Poluboyarova Tatiana V. Poluboyarova Tatiana V. Poluboyarova Gennady V. Vasiliev Elena V. Golovanova Anna P. Geraskina Nina A. Bulakhova Nina A. Bulakhova Tímea Szederjesi Sergei E. Peltek Sergei E. Peltek |
| author_sort |
Sergei V. Shekhovtsov |
| title |
Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data |
| title_short |
Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data |
| title_full |
Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data |
| title_fullStr |
Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data |
| title_full_unstemmed |
Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data |
| title_sort |
species delimitation of the eisenia nordenskioldi complex (oligochaeta, lumbricidae) using transcriptomic data |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Genetics |
| issn |
1664-8021 |
| publishDate |
2020-12-01 |
| description |
Eisenia nordenskioldi (Eisen, 1879) is the only autochthonous Siberian earthworm with a large distribution that ranges from tundra to steppe and broadleaved forests. This species has a very high morphological, ecological, karyological, and genetic diversity, so it was proposed that E. nordenskioldi should be split into several species. However, the phylogeny of the complex was unclear due to the low resolution of the methods used and the high diversity that should have been taken into account. We investigated this question by (1) studying the diversity of the COI gene of E. nordenskioldi throughout its range and (2) sequencing transcriptomes of different genetic lineages to infer its phylogeny. We found that E. nordenskioldi is monophyletic and is split into two clades. The first one includes the pigmented genetic lineages widespread in the northern and western parts of the distribution, and the second one originating from the southern and southeastern part of the species' range and representing both pigmented and non-pigmented forms. We propose to split the E. nordenskioldi complex into two species, E. nordenskioldi and Eisenia sp. 1 (aff. E. nordenskioldi), corresponding to these two clades. The currently recognized non-pigmented subspecies E. n. pallida will be abolished as a polyphyletic and thus a non-natural taxon, while Eisenia sp. 1 will be expanded to include several lineages earlier recognized as E. n. nordenskioldi and E. n. pallida. |
| topic |
phylogenomics phylogeny transcriptomes Lumbricidae Eisenia nordenskioldi earthworms |
| url |
https://www.frontiersin.org/articles/10.3389/fgene.2020.598196/full |
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doaj-00b3f3b0153b40c395a894cd52e170d52020-12-08T08:33:30ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-12-011110.3389/fgene.2020.598196598196Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic DataSergei V. Shekhovtsov0Sergei V. Shekhovtsov1Sergei V. Shekhovtsov2Aleksandra A. Shipova3Aleksandra A. Shipova4Tatiana V. Poluboyarova5Tatiana V. Poluboyarova6Tatiana V. Poluboyarova7Gennady V. Vasiliev8Elena V. Golovanova9Anna P. Geraskina10Nina A. Bulakhova11Nina A. Bulakhova12Tímea Szederjesi13Sergei E. Peltek14Sergei E. Peltek15Department of Molecular Biotechnology, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaKurchatov Genomic Center, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaLaboratory of Biocenology, Institute of Biological Problems of the North of the Far Eastern Branch of the Russian Academy of Sciences, Magadan, RussiaDepartment of Molecular Biotechnology, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaKurchatov Genomic Center, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaDepartment of Molecular Biotechnology, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaKurchatov Genomic Center, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaLaboratory of Biocenology, Institute of Biological Problems of the North of the Far Eastern Branch of the Russian Academy of Sciences, Magadan, RussiaDepartment of Molecular Biotechnology, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaLaboratory of Systematics and Ecology of Invertebrates, Omsk State Pedagogical University, Omsk, RussiaCenter for Forest Ecology and Productivity of the Russian Academy of Sciences, Moscow, RussiaLaboratory of Biocenology, Institute of Biological Problems of the North of the Far Eastern Branch of the Russian Academy of Sciences, Magadan, RussiaLaboratory of Biodiversity and Ecology, Tomsk State University, Tomsk, RussiaDepartment of Zoology, Hungarian Natural History Museum, Budapest, HungaryDepartment of Molecular Biotechnology, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaKurchatov Genomic Center, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaEisenia nordenskioldi (Eisen, 1879) is the only autochthonous Siberian earthworm with a large distribution that ranges from tundra to steppe and broadleaved forests. This species has a very high morphological, ecological, karyological, and genetic diversity, so it was proposed that E. nordenskioldi should be split into several species. However, the phylogeny of the complex was unclear due to the low resolution of the methods used and the high diversity that should have been taken into account. We investigated this question by (1) studying the diversity of the COI gene of E. nordenskioldi throughout its range and (2) sequencing transcriptomes of different genetic lineages to infer its phylogeny. We found that E. nordenskioldi is monophyletic and is split into two clades. The first one includes the pigmented genetic lineages widespread in the northern and western parts of the distribution, and the second one originating from the southern and southeastern part of the species' range and representing both pigmented and non-pigmented forms. We propose to split the E. nordenskioldi complex into two species, E. nordenskioldi and Eisenia sp. 1 (aff. E. nordenskioldi), corresponding to these two clades. The currently recognized non-pigmented subspecies E. n. pallida will be abolished as a polyphyletic and thus a non-natural taxon, while Eisenia sp. 1 will be expanded to include several lineages earlier recognized as E. n. nordenskioldi and E. n. pallida.https://www.frontiersin.org/articles/10.3389/fgene.2020.598196/fullphylogenomicsphylogenytranscriptomesLumbricidaeEisenia nordenskioldiearthworms |