An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma
Abstract Background ESPN (Espin), an actin filament-binding protein, plays an important role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and che...
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doaj-00b48016dbc847d488699e7013dddafd2020-11-25T02:09:36ZengBMCCancer Cell International1475-28672018-12-011811910.1186/s12935-018-0713-xAn actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinomaShau-Hsuan Li0Hung-I Lu1Wan-Ting Huang2Yen-Hao Chen3Chien-Ming Lo4Ya-Chun Lan5Wei-Che Lin6Hsin-Ting Tsai7Chang-Han Chen8Department of Hematology-Oncology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Thoracic & Cardiovascular Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Pathology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Hematology-Oncology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Thoracic & Cardiovascular Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Hematology-Oncology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Diagnostic Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Applied Chemistry, and Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan UniversityDepartment of Applied Chemistry, and Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan UniversityAbstract Background ESPN (Espin), an actin filament-binding protein, plays an important role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Recent few studies show that ESPN regulates metastasis and cell proliferation in melanoma. However, the significance of ESPN in other cancers such as esophageal squamous cell carcinoma (ESCC) remains largely unknown. Methods Immunohistochemistry was performed in 169 patients with ESCC and correlated with clinicopathological features and survival. The functional role of ESPN in ESCC cells was determined by ESPN-mediated siRNA. Results Univariate analyses showed that high ESPN expression was associated with inferior overall survival (P = 0.005) and disease-free survival (P = 0.035). High ESPN expression was an independent prognosticator in multivariate analysis for overall survival (P = 0.009, hazard ratio = 1.688) and disease-free survival (P = 0.049, hazard ratio = 1.451). The 5-year overall survival rates were 30% and 54% in patients with high and low expression of ESPN, respectively. Inhibition of endogenous ESPN in ESCC cells decreased ESCC growth by reducing cell proliferating rates. Conclusions High ESPN expression is independently associated with poor prognosis in patients with ESCC and downregulation of ESPN inhibits ESCC cell growth. Our results suggest that ESPN may be a novel therapeutic target for patients with ESCC.http://link.springer.com/article/10.1186/s12935-018-0713-xEsophageal cancerSquamous cell carcinomaESPN |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shau-Hsuan Li Hung-I Lu Wan-Ting Huang Yen-Hao Chen Chien-Ming Lo Ya-Chun Lan Wei-Che Lin Hsin-Ting Tsai Chang-Han Chen |
spellingShingle |
Shau-Hsuan Li Hung-I Lu Wan-Ting Huang Yen-Hao Chen Chien-Ming Lo Ya-Chun Lan Wei-Che Lin Hsin-Ting Tsai Chang-Han Chen An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma Cancer Cell International Esophageal cancer Squamous cell carcinoma ESPN |
author_facet |
Shau-Hsuan Li Hung-I Lu Wan-Ting Huang Yen-Hao Chen Chien-Ming Lo Ya-Chun Lan Wei-Che Lin Hsin-Ting Tsai Chang-Han Chen |
author_sort |
Shau-Hsuan Li |
title |
An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma |
title_short |
An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma |
title_full |
An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma |
title_fullStr |
An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma |
title_full_unstemmed |
An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma |
title_sort |
actin-binding protein espn is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2018-12-01 |
description |
Abstract Background ESPN (Espin), an actin filament-binding protein, plays an important role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Recent few studies show that ESPN regulates metastasis and cell proliferation in melanoma. However, the significance of ESPN in other cancers such as esophageal squamous cell carcinoma (ESCC) remains largely unknown. Methods Immunohistochemistry was performed in 169 patients with ESCC and correlated with clinicopathological features and survival. The functional role of ESPN in ESCC cells was determined by ESPN-mediated siRNA. Results Univariate analyses showed that high ESPN expression was associated with inferior overall survival (P = 0.005) and disease-free survival (P = 0.035). High ESPN expression was an independent prognosticator in multivariate analysis for overall survival (P = 0.009, hazard ratio = 1.688) and disease-free survival (P = 0.049, hazard ratio = 1.451). The 5-year overall survival rates were 30% and 54% in patients with high and low expression of ESPN, respectively. Inhibition of endogenous ESPN in ESCC cells decreased ESCC growth by reducing cell proliferating rates. Conclusions High ESPN expression is independently associated with poor prognosis in patients with ESCC and downregulation of ESPN inhibits ESCC cell growth. Our results suggest that ESPN may be a novel therapeutic target for patients with ESCC. |
topic |
Esophageal cancer Squamous cell carcinoma ESPN |
url |
http://link.springer.com/article/10.1186/s12935-018-0713-x |
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