Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervical Carcinoma (C33-A)

• Main Authors: Mirandeli Bautista, Ángel Morales-González, Roberto Villagómez-Ibarra, Cesar Esquivel-Chirino, Jaime Esquivel-Soto, José A. Morales-González, Clara Zuñiga-Pérez, Eduardo Madrigal-Santillán, Leticia Riverón-Negrete, Alejandro García-Carrancá, Carmen Valadez-Vega, Gerardo Alvarez-Manilla
• Format: Article
• Language: English
• Published: MDPI AG 2011-03-01
• Series: Molecules
• Subjects: lectins; tepary beans; cytotoxicity; tepary bean lectins
• Online Access: http://www.mdpi.com/1420-3049/16/3/2107/

Lectins comprise a heterogeneous class of proteins that recognize the carbohydrate moieties of glycoconjugates with high specificity. Numerous studies have shown that lectins are capable of recognizing specific carbohydrate moieties displayed by malignant cells or tissues. The present work was performed to investigate the effects of tepary bean (Phaseolus acutifolius) lectins on proliferation, colony formation, and alteration of DNA synthesis of human malignant cells. Tepary bean lectin showed dose dependent  effects on the inhibition of viability as well as on colony formation in two human malignant cells lines (C33-A, Sw480); By contrast, tepary bean lectin only showed significant effects on DNA synthesis on Sw480 cells. Our results provide evidence of the anti- proliferative and cytotoxic effects of the tepary bean lectins on C33-A and Sw480 cells lines.