Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis
Background& Objective: Candidal vulvovaginitis occurs in female genital by the over growth of candida especially candida albicans. This infection may be resistant to therapy and occasionally becomes chronic. In some patients, this form of infection is recurrent. Moreover, discovering n...
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doaj-00c6e24bc8e14318be50d8d09a02b03d2020-11-24T21:23:48ZfasFasa University of Medical SciencesJournal of Fasa University of Medical Sciences2228-51052228-73292014-11-0143327334Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal VulvovaginitisEfate alsadat shojaei0Mehraban Falahati1Faride zaini2Parivash kord bache3Parvaneh rahimi moghadam 4Mohammad reza aghamirian5Shima Nozari6Ayesheh Makhdoomi 7Sekhavatv ameri8Sanam afshar moghadam 9 Department of Parasitology and Mycology, Iran University of Medical Sciences, Tehran, Iran. Department of Parasitology and Mycology, Iran University of Medical Sciences, Tehran, Iran. Department of Parasitology and Mycology, Iran University of Medical Sciences, Tehran, Iran. Department of Parasitology and Mycology, Iran University of Medical Sciences, Tehran, Iran. Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran. Department of Parasitology and Mycology, Qazvin University of Medical Sciences. Department of Parasitology and Mycology, Bandar Abbas University of Medical sciences Department of Parasitology and Mycology, Qazvin University of Medical Sciences. Department of Parasitology and Mycology, Iran University of Medical Sciences, Tehran, Iran. Department of Parasitology and Mycology, Iran University of Medical Sciences, Tehran, Iran. Background& Objective: Candidal vulvovaginitis occurs in female genital by the over growth of candida especially candida albicans. This infection may be resistant to therapy and occasionally becomes chronic. In some patients, this form of infection is recurrent. Moreover, discovering new antifungal drugs and the use of these drugs in new methods encourage mycologists to use these techniques. Therefore, according to the above methods, this research investigated effects of several common antifungal drugs with the use of combination methods against candida species isolated from chronic candidal vulvovaginitis.Materials& Methods: This study carried out on 19 strains of candida albicans that were isolated from recurrent vulvovaginitis. The effects of clotrimazole, miconazole, fluconazole were assessed separately and in combination with amphotericin B on candida albicans using micro dilution methods.Results: The mean Minimum Inhibitory Concentration (MIC) of clotrimazole, miconazole, fluconazole, and amphotericin B were 7.05, 10.7,47, and 0.6 µgr/ml, respectively after 48 hours incubating. Then, the azole antifungal drugs were evaluated in combination with amphotericin B. The two combinations of fluconazole + amphotericin and miconazole + amphotericin with the Fungicidal Inhibitory Concentrations (FIC) of 0.6 and 1, respectively, attracted the most attentions. In addition, comparing the antifungal effects, the most effective drug was amphotericin B with the Minimum Fungicidal Concentration (MFC) of 1.18.Conclusion: In this study, amphotericin B with the least MIC had the best antifungal effect. The most and the least synergic effects of combination therapy were for fluconazole + amphotericin B and Miconazole + amphotericin B, respectively.http://journal.fums.ac.ir/browse.php?a_code=A-10-29-2&slc_lang=en&sid=1Chronic candidal vulvovaginitis Drugs combination Synergism Micro dilution broth Clotrimazole Miconazole Fluconazole Amphotericin B |
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DOAJ |
author |
Efate alsadat shojaei Mehraban Falahati Faride zaini Parivash kord bache Parvaneh rahimi moghadam Mohammad reza aghamirian Shima Nozari Ayesheh Makhdoomi Sekhavatv ameri Sanam afshar moghadam |
spellingShingle |
Efate alsadat shojaei Mehraban Falahati Faride zaini Parivash kord bache Parvaneh rahimi moghadam Mohammad reza aghamirian Shima Nozari Ayesheh Makhdoomi Sekhavatv ameri Sanam afshar moghadam Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis Journal of Fasa University of Medical Sciences Chronic candidal vulvovaginitis Drugs combination Synergism Micro dilution broth Clotrimazole Miconazole Fluconazole Amphotericin B |
author_facet |
Efate alsadat shojaei Mehraban Falahati Faride zaini Parivash kord bache Parvaneh rahimi moghadam Mohammad reza aghamirian Shima Nozari Ayesheh Makhdoomi Sekhavatv ameri Sanam afshar moghadam |
author_sort |
Efate alsadat shojaei |
title |
Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis |
title_short |
Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis |
title_full |
Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis |
title_fullStr |
Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis |
title_full_unstemmed |
Effects of Several Common Antifungal Drugs (Clotrimazole, Miconazole, Fluconazole) alone and in Combination with Amphotericin B on Candida Species Isolated from Chronic Candidal Vulvovaginitis |
title_sort |
effects of several common antifungal drugs (clotrimazole, miconazole, fluconazole) alone and in combination with amphotericin b on candida species isolated from chronic candidal vulvovaginitis |
publisher |
Fasa University of Medical Sciences |
series |
Journal of Fasa University of Medical Sciences |
issn |
2228-5105 2228-7329 |
publishDate |
2014-11-01 |
description |
Background&
Objective: Candidal vulvovaginitis
occurs in female genital by the over growth of candida especially candida
albicans. This infection may be resistant to therapy and occasionally becomes
chronic. In some patients, this form of infection is recurrent. Moreover,
discovering new antifungal drugs and the use of these drugs in new methods
encourage mycologists to use these techniques. Therefore, according to the
above methods, this research investigated effects of several common antifungal
drugs with the use of combination methods against candida species isolated from
chronic candidal vulvovaginitis.Materials&
Methods: This study carried out
on 19 strains of candida albicans that were isolated from recurrent
vulvovaginitis. The effects of clotrimazole, miconazole, fluconazole were
assessed separately and in combination with amphotericin B on candida albicans
using micro dilution methods.Results: The mean Minimum Inhibitory Concentration (MIC)
of clotrimazole, miconazole, fluconazole, and amphotericin B were 7.05,
10.7,47, and 0.6 µgr/ml, respectively after 48 hours incubating. Then, the
azole antifungal drugs were evaluated in combination with amphotericin B. The
two combinations of fluconazole + amphotericin and miconazole + amphotericin
with the Fungicidal Inhibitory Concentrations (FIC) of 0.6 and 1, respectively,
attracted the most attentions. In addition, comparing the antifungal effects,
the most effective drug was amphotericin B with the Minimum Fungicidal
Concentration (MFC) of 1.18.Conclusion: In this study, amphotericin B with the least
MIC had the best antifungal effect. The most and the least synergic effects of
combination therapy were for fluconazole + amphotericin B and Miconazole +
amphotericin B, respectively. |
topic |
Chronic candidal vulvovaginitis Drugs combination Synergism Micro dilution broth Clotrimazole Miconazole Fluconazole Amphotericin B |
url |
http://journal.fums.ac.ir/browse.php?a_code=A-10-29-2&slc_lang=en&sid=1 |
work_keys_str_mv |
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