Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR‐578
Abstract Background Circular RNAs (circRNAs) had been identified as a non‐coding RNA associated with many types of cancer in recent years. However, the involvement of hsa_circ_0008274 in lung adenocarcinoma (LUAD) has not been explored. The aim of our research is to explore the biological mechanism...
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doaj-00cbd924130b4322a7eea80cd2ddf50c2021-08-16T04:32:26ZengWileyThoracic Cancer1759-77061759-77142021-08-0112162258226410.1111/1759-7714.14059Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR‐578Maolong Wang0Minge Ma1Yuling Yang2Chuan Li3Yuanyong Wang4Xiao Sun5Mengdi Wang6Yong Sun7Wenjie Jiao8Department of Thoracic Surgery The Affiliated Hospital of Qingdao University Qingdao ChinaDepartment of Radiology The Affiliated Hospital of Qingdao University Qingdao ChinaDepartment of Infectious diseases The Affiliated Hospital of Qingdao University Qingdao ChinaDepartment of Thoracic Surgery The Affiliated Hospital of Qingdao University Qingdao ChinaDepartment of Thoracic Surgery The Affiliated Hospital of Qingdao University Qingdao ChinaDepartment of Thoracic Surgery The Affiliated Hospital of Qingdao University Qingdao ChinaDepartment of Pharmacology School of Pharmacy, Qingdao University Qingdao ChinaDepartment of Pharmacology School of Pharmacy, Qingdao University Qingdao ChinaDepartment of Thoracic Surgery The Affiliated Hospital of Qingdao University Qingdao ChinaAbstract Background Circular RNAs (circRNAs) had been identified as a non‐coding RNA associated with many types of cancer in recent years. However, the involvement of hsa_circ_0008274 in lung adenocarcinoma (LUAD) has not been explored. The aim of our research is to explore the biological mechanism and function of hsa_circ_0008274 in LUAD. Methods The hsa_circ_0008274, miR‐578, and high mobility group AT‐Hook 2 (HMGA2) mRNA expression levels were detected via qRT‐PCR. Cell Counting Kit‐8 (CCK‐8) Transwell assay and wound healing assay were performed to measure the cell proliferation, invasion, and migration ability. Luciferase reporter and Western blotting experiments were performed to characterize the competing endogenous RNA (ceRNA) mechanism of hsa_circ_0008274. Results Our findings determined that the expression of hsa_circ_0008274 in LUAD was significantly decreased. Cell experiments showed that overexpressed hsa_circ_0008274 could reduce the proliferation and invasion ability of LUAD cells. Moreover, miRNA‐578 could identify as a miRNA sponge of hsa_circ_0008274. Overexpressed hsa_circ_0008274 reduced the proliferation and invasion of LUAD cells caused by miR‐578 mimics. Increasing the expression of miR‐578 can aggravate the proliferation and invasion of LUAD cells and block the inhibition of proliferation and invasion of LUAD cells mediated by overexpressed hsa_circ_0008274. Subsequent data indicate that HMGA2 of the tumor‐promoting gene is the target gene of miR‐578. The upregulation of HMGA2 partially reversed the tumor inhibitory effect of LUAD cells induced by overexpressed hsa_circ_0008274 or miR‐578 mimics. Conclusions In summary, our data show that the overexpression of hsa_circ_0008274 repressed the proliferation and invasion of LUAD through downregulating miR‐578 and activating HMGA2.https://doi.org/10.1111/1759-7714.14059HMGA2hsa_circ_0008274lung adenocarcinomamiR‐578 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maolong Wang Minge Ma Yuling Yang Chuan Li Yuanyong Wang Xiao Sun Mengdi Wang Yong Sun Wenjie Jiao |
spellingShingle |
Maolong Wang Minge Ma Yuling Yang Chuan Li Yuanyong Wang Xiao Sun Mengdi Wang Yong Sun Wenjie Jiao Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR‐578 Thoracic Cancer HMGA2 hsa_circ_0008274 lung adenocarcinoma miR‐578 |
author_facet |
Maolong Wang Minge Ma Yuling Yang Chuan Li Yuanyong Wang Xiao Sun Mengdi Wang Yong Sun Wenjie Jiao |
author_sort |
Maolong Wang |
title |
Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR‐578 |
title_short |
Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR‐578 |
title_full |
Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR‐578 |
title_fullStr |
Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR‐578 |
title_full_unstemmed |
Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR‐578 |
title_sort |
overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating hmga2 via sponging mir‐578 |
publisher |
Wiley |
series |
Thoracic Cancer |
issn |
1759-7706 1759-7714 |
publishDate |
2021-08-01 |
description |
Abstract Background Circular RNAs (circRNAs) had been identified as a non‐coding RNA associated with many types of cancer in recent years. However, the involvement of hsa_circ_0008274 in lung adenocarcinoma (LUAD) has not been explored. The aim of our research is to explore the biological mechanism and function of hsa_circ_0008274 in LUAD. Methods The hsa_circ_0008274, miR‐578, and high mobility group AT‐Hook 2 (HMGA2) mRNA expression levels were detected via qRT‐PCR. Cell Counting Kit‐8 (CCK‐8) Transwell assay and wound healing assay were performed to measure the cell proliferation, invasion, and migration ability. Luciferase reporter and Western blotting experiments were performed to characterize the competing endogenous RNA (ceRNA) mechanism of hsa_circ_0008274. Results Our findings determined that the expression of hsa_circ_0008274 in LUAD was significantly decreased. Cell experiments showed that overexpressed hsa_circ_0008274 could reduce the proliferation and invasion ability of LUAD cells. Moreover, miRNA‐578 could identify as a miRNA sponge of hsa_circ_0008274. Overexpressed hsa_circ_0008274 reduced the proliferation and invasion of LUAD cells caused by miR‐578 mimics. Increasing the expression of miR‐578 can aggravate the proliferation and invasion of LUAD cells and block the inhibition of proliferation and invasion of LUAD cells mediated by overexpressed hsa_circ_0008274. Subsequent data indicate that HMGA2 of the tumor‐promoting gene is the target gene of miR‐578. The upregulation of HMGA2 partially reversed the tumor inhibitory effect of LUAD cells induced by overexpressed hsa_circ_0008274 or miR‐578 mimics. Conclusions In summary, our data show that the overexpression of hsa_circ_0008274 repressed the proliferation and invasion of LUAD through downregulating miR‐578 and activating HMGA2. |
topic |
HMGA2 hsa_circ_0008274 lung adenocarcinoma miR‐578 |
url |
https://doi.org/10.1111/1759-7714.14059 |
work_keys_str_mv |
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