Intra-Abdominal Lipopolysaccharide Clearance and Inactivation in Peritonitis: Key Roles for Lipoproteins and the Phospholipid Transfer Protein
IntroductionDuring peritonitis, lipopolysaccharides (LPS) cross the peritoneum and pass through the liver before reaching the central compartment. The aim of the present study was to investigate the role of lipoproteins and phospholipid transfer protein (PLTP) in the early stages of LPS detoxificati...
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Frontiers Media S.A.
2021-05-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.622935/full |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Maxime Nguyen Maxime Nguyen Maxime Nguyen Maxime Nguyen Gaëtan Pallot Gaëtan Pallot Antoine Jalil Antoine Jalil Annabelle Tavernier Annabelle Tavernier Aloïs Dusuel Aloïs Dusuel Naig Le Guern Naig Le Guern Laurent Lagrost Laurent Lagrost Laurent Lagrost Jean-Paul Pais de Barros Jean-Paul Pais de Barros Hélène Choubley Hélène Choubley Victoria Bergas Victoria Bergas Pierre-Grégoire Guinot Pierre-Grégoire Guinot Pierre-Grégoire Guinot Pierre-Grégoire Guinot David Masson David Masson David Masson David Masson Belaid Bouhemad Belaid Bouhemad Belaid Bouhemad Belaid Bouhemad Thomas Gautier Thomas Gautier Thomas Gautier |
spellingShingle |
Maxime Nguyen Maxime Nguyen Maxime Nguyen Maxime Nguyen Gaëtan Pallot Gaëtan Pallot Antoine Jalil Antoine Jalil Annabelle Tavernier Annabelle Tavernier Aloïs Dusuel Aloïs Dusuel Naig Le Guern Naig Le Guern Laurent Lagrost Laurent Lagrost Laurent Lagrost Jean-Paul Pais de Barros Jean-Paul Pais de Barros Hélène Choubley Hélène Choubley Victoria Bergas Victoria Bergas Pierre-Grégoire Guinot Pierre-Grégoire Guinot Pierre-Grégoire Guinot Pierre-Grégoire Guinot David Masson David Masson David Masson David Masson Belaid Bouhemad Belaid Bouhemad Belaid Bouhemad Belaid Bouhemad Thomas Gautier Thomas Gautier Thomas Gautier Intra-Abdominal Lipopolysaccharide Clearance and Inactivation in Peritonitis: Key Roles for Lipoproteins and the Phospholipid Transfer Protein Frontiers in Immunology endotoxemia peritonitis sepsis phospholipid transfer protein lipoproteins inflammation |
author_facet |
Maxime Nguyen Maxime Nguyen Maxime Nguyen Maxime Nguyen Gaëtan Pallot Gaëtan Pallot Antoine Jalil Antoine Jalil Annabelle Tavernier Annabelle Tavernier Aloïs Dusuel Aloïs Dusuel Naig Le Guern Naig Le Guern Laurent Lagrost Laurent Lagrost Laurent Lagrost Jean-Paul Pais de Barros Jean-Paul Pais de Barros Hélène Choubley Hélène Choubley Victoria Bergas Victoria Bergas Pierre-Grégoire Guinot Pierre-Grégoire Guinot Pierre-Grégoire Guinot Pierre-Grégoire Guinot David Masson David Masson David Masson David Masson Belaid Bouhemad Belaid Bouhemad Belaid Bouhemad Belaid Bouhemad Thomas Gautier Thomas Gautier Thomas Gautier |
author_sort |
Maxime Nguyen |
title |
Intra-Abdominal Lipopolysaccharide Clearance and Inactivation in Peritonitis: Key Roles for Lipoproteins and the Phospholipid Transfer Protein |
title_short |
Intra-Abdominal Lipopolysaccharide Clearance and Inactivation in Peritonitis: Key Roles for Lipoproteins and the Phospholipid Transfer Protein |
title_full |
Intra-Abdominal Lipopolysaccharide Clearance and Inactivation in Peritonitis: Key Roles for Lipoproteins and the Phospholipid Transfer Protein |
title_fullStr |
Intra-Abdominal Lipopolysaccharide Clearance and Inactivation in Peritonitis: Key Roles for Lipoproteins and the Phospholipid Transfer Protein |
title_full_unstemmed |
Intra-Abdominal Lipopolysaccharide Clearance and Inactivation in Peritonitis: Key Roles for Lipoproteins and the Phospholipid Transfer Protein |
title_sort |
intra-abdominal lipopolysaccharide clearance and inactivation in peritonitis: key roles for lipoproteins and the phospholipid transfer protein |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2021-05-01 |
description |
IntroductionDuring peritonitis, lipopolysaccharides (LPS) cross the peritoneum and pass through the liver before reaching the central compartment. The aim of the present study was to investigate the role of lipoproteins and phospholipid transfer protein (PLTP) in the early stages of LPS detoxification.Material and MethodsPeritonitis was induced by intra-peritoneal injection of LPS in mice. We analyzed peritoneal fluid, portal and central blood. Lipoprotein fractions were obtained by ultracentrifugation and fast protein liquid chromatography. LPS concentration and activity were measured by liquid chromatography coupled with mass spectrometry and limulus amoebocyte lysate. Wild-type mice were compared to mice knocked out for PLTP.ResultsIn mice expressing PLTP, LPS was able to bind to HDL in the peritoneal compartment, and this was maintained in plasma from portal and central blood. A hepatic first-pass effect of HDL-bound LPS was observed in wild-type mice. LPS binding to HDL resulted in an early arrival of inactive LPS in the central blood of wild-type mice.ConclusionPLTP promotes LPS peritoneal clearance and neutralization in a model of peritonitis. This mechanism involves the early binding of LPS to lipoproteins inside the peritoneal cavity, which promotes LPS translocation through the peritoneum and its uptake by the liver. |
topic |
endotoxemia peritonitis sepsis phospholipid transfer protein lipoproteins inflammation |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.622935/full |
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doaj-00d3c73725f8440f8c39f3fb958c5b012021-05-12T05:49:59ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-05-011210.3389/fimmu.2021.622935622935Intra-Abdominal Lipopolysaccharide Clearance and Inactivation in Peritonitis: Key Roles for Lipoproteins and the Phospholipid Transfer ProteinMaxime Nguyen0Maxime Nguyen1Maxime Nguyen2Maxime Nguyen3Gaëtan Pallot4Gaëtan Pallot5Antoine Jalil6Antoine Jalil7Annabelle Tavernier8Annabelle Tavernier9Aloïs Dusuel10Aloïs Dusuel11Naig Le Guern12Naig Le Guern13Laurent Lagrost14Laurent Lagrost15Laurent Lagrost16Jean-Paul Pais de Barros17Jean-Paul Pais de Barros18Hélène Choubley19Hélène Choubley20Victoria Bergas21Victoria Bergas22Pierre-Grégoire Guinot23Pierre-Grégoire Guinot24Pierre-Grégoire Guinot25Pierre-Grégoire Guinot26David Masson27David Masson28David Masson29David Masson30Belaid Bouhemad31Belaid Bouhemad32Belaid Bouhemad33Belaid Bouhemad34Thomas Gautier35Thomas Gautier36Thomas Gautier37Department of Anesthesiology and Intensive Care, Dijon University Hospital, Dijon, FranceUniversité Bourgogne Franche-Comté / Agrosup, Lipids Nutrition Cancer (LNC) UMR1231, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceUniversité Bourgogne Franche-Comté / Agrosup, Lipids Nutrition Cancer (LNC) UMR1231, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceLipidomic Analytical Platform, Université Bourgogne Franche-Comté (UBFC), Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceLipidomic Analytical Platform, Université Bourgogne Franche-Comté (UBFC), Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceLipidomic Analytical Platform, Université Bourgogne Franche-Comté (UBFC), Dijon, FranceDepartment of Anesthesiology and Intensive Care, Dijon University Hospital, Dijon, FranceUniversité Bourgogne Franche-Comté / Agrosup, Lipids Nutrition Cancer (LNC) UMR1231, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceUniversité Bourgogne Franche-Comté / Agrosup, Lipids Nutrition Cancer (LNC) UMR1231, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceLaboratory of Clinical Chemistry, François Mitterrand University Hospital, Dijon, FranceDepartment of Anesthesiology and Intensive Care, Dijon University Hospital, Dijon, FranceUniversité Bourgogne Franche-Comté / Agrosup, Lipids Nutrition Cancer (LNC) UMR1231, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceUniversité Bourgogne Franche-Comté / Agrosup, Lipids Nutrition Cancer (LNC) UMR1231, Dijon, FranceINSERM, LNC UMR1231, Dijon, FranceFCS Bourgogne-Franche Comté, LipSTIC LabEx, Dijon, FranceIntroductionDuring peritonitis, lipopolysaccharides (LPS) cross the peritoneum and pass through the liver before reaching the central compartment. The aim of the present study was to investigate the role of lipoproteins and phospholipid transfer protein (PLTP) in the early stages of LPS detoxification.Material and MethodsPeritonitis was induced by intra-peritoneal injection of LPS in mice. We analyzed peritoneal fluid, portal and central blood. Lipoprotein fractions were obtained by ultracentrifugation and fast protein liquid chromatography. LPS concentration and activity were measured by liquid chromatography coupled with mass spectrometry and limulus amoebocyte lysate. Wild-type mice were compared to mice knocked out for PLTP.ResultsIn mice expressing PLTP, LPS was able to bind to HDL in the peritoneal compartment, and this was maintained in plasma from portal and central blood. A hepatic first-pass effect of HDL-bound LPS was observed in wild-type mice. LPS binding to HDL resulted in an early arrival of inactive LPS in the central blood of wild-type mice.ConclusionPLTP promotes LPS peritoneal clearance and neutralization in a model of peritonitis. This mechanism involves the early binding of LPS to lipoproteins inside the peritoneal cavity, which promotes LPS translocation through the peritoneum and its uptake by the liver.https://www.frontiersin.org/articles/10.3389/fimmu.2021.622935/fullendotoxemiaperitonitissepsisphospholipid transfer proteinlipoproteinsinflammation |