Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on <i>Streptococcus mutans</i>
There is no consensus on the antibacterial activity of dentin bonding systems (DBS). Many study models have been used to evaluate the antimicrobial activity of dental materials. In this study, a novel detection method, flow cytometry, was introduced. It allows for evaluation of the antibacterial act...
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doaj-00d73f88248a46d78c6415b91c2569372020-11-24T20:40:18ZengMDPI AGMolecules1420-30492019-02-0124353210.3390/molecules24030532molecules24030532Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on <i>Streptococcus mutans</i>Barbara Lapinska0Magdalena Konieczka1Beata Zarzycka2Krzysztof Sokolowski3Janina Grzegorczyk4Monika Lukomska-Szymanska5Department of General Dentistry, Medical University of Lodz, 92-213 Lodz, PolandDepartment of Microbiology and Laboratory Medical Immunology, Medical University of Lodz, 92-213 Lodz, PolandDepartment of Microbiology and Laboratory Medical Immunology, Medical University of Lodz, 92-213 Lodz, PolandDepartment of Conservative Dentistry, Medical University of Lodz, 92-213 Lodz, PolandDepartment of Microbiology and Laboratory Medical Immunology, Medical University of Lodz, 92-213 Lodz, PolandDepartment of General Dentistry, Medical University of Lodz, 92-213 Lodz, PolandThere is no consensus on the antibacterial activity of dentin bonding systems (DBS). Many study models have been used to evaluate the antimicrobial activity of dental materials. In this study, a novel detection method, flow cytometry, was introduced. It allows for evaluation of the antibacterial activity of DBS, based on assessment of the disruption of the bacterial physical membrane induced by DBS. The aim of the study was to evaluate the antibacterial properties of selected dentin bonding systems against <i>Streptococcus mutans</i>. The highest antibacterial activity against <i>S. mutans</i> was observed for Adhese Universal (99.68% dead cells) and was comparable to that of Prime&Bond Universal, OptiBond Universal, or Clearfil Universal Bond Quick (<i>p</i> > 0.05). The lowest activity of all tested systems was displayed by the multi-mode adhesive, Universal Bond (12.68% dead bacteria cells), followed by the self-etch adhesive, OptiBond FL (15.58% dead bacteria cells). The present study showed that in the case of two-component DBS, the primer exhibited higher antimicrobial activity than the adhesive (or bond) itself.https://www.mdpi.com/1420-3049/24/3/532dental bonding systemflow cytometryantibacterial activity<i>S. mutans</i>multi-mode adhesive |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Barbara Lapinska Magdalena Konieczka Beata Zarzycka Krzysztof Sokolowski Janina Grzegorczyk Monika Lukomska-Szymanska |
spellingShingle |
Barbara Lapinska Magdalena Konieczka Beata Zarzycka Krzysztof Sokolowski Janina Grzegorczyk Monika Lukomska-Szymanska Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on <i>Streptococcus mutans</i> Molecules dental bonding system flow cytometry antibacterial activity <i>S. mutans</i> multi-mode adhesive |
author_facet |
Barbara Lapinska Magdalena Konieczka Beata Zarzycka Krzysztof Sokolowski Janina Grzegorczyk Monika Lukomska-Szymanska |
author_sort |
Barbara Lapinska |
title |
Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on <i>Streptococcus mutans</i> |
title_short |
Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on <i>Streptococcus mutans</i> |
title_full |
Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on <i>Streptococcus mutans</i> |
title_fullStr |
Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on <i>Streptococcus mutans</i> |
title_full_unstemmed |
Flow Cytometry Analysis of Antibacterial Effects of Universal Dentin Bonding Agents on <i>Streptococcus mutans</i> |
title_sort |
flow cytometry analysis of antibacterial effects of universal dentin bonding agents on <i>streptococcus mutans</i> |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-02-01 |
description |
There is no consensus on the antibacterial activity of dentin bonding systems (DBS). Many study models have been used to evaluate the antimicrobial activity of dental materials. In this study, a novel detection method, flow cytometry, was introduced. It allows for evaluation of the antibacterial activity of DBS, based on assessment of the disruption of the bacterial physical membrane induced by DBS. The aim of the study was to evaluate the antibacterial properties of selected dentin bonding systems against <i>Streptococcus mutans</i>. The highest antibacterial activity against <i>S. mutans</i> was observed for Adhese Universal (99.68% dead cells) and was comparable to that of Prime&Bond Universal, OptiBond Universal, or Clearfil Universal Bond Quick (<i>p</i> > 0.05). The lowest activity of all tested systems was displayed by the multi-mode adhesive, Universal Bond (12.68% dead bacteria cells), followed by the self-etch adhesive, OptiBond FL (15.58% dead bacteria cells). The present study showed that in the case of two-component DBS, the primer exhibited higher antimicrobial activity than the adhesive (or bond) itself. |
topic |
dental bonding system flow cytometry antibacterial activity <i>S. mutans</i> multi-mode adhesive |
url |
https://www.mdpi.com/1420-3049/24/3/532 |
work_keys_str_mv |
AT barbaralapinska flowcytometryanalysisofantibacterialeffectsofuniversaldentinbondingagentsonistreptococcusmutansi AT magdalenakonieczka flowcytometryanalysisofantibacterialeffectsofuniversaldentinbondingagentsonistreptococcusmutansi AT beatazarzycka flowcytometryanalysisofantibacterialeffectsofuniversaldentinbondingagentsonistreptococcusmutansi AT krzysztofsokolowski flowcytometryanalysisofantibacterialeffectsofuniversaldentinbondingagentsonistreptococcusmutansi AT janinagrzegorczyk flowcytometryanalysisofantibacterialeffectsofuniversaldentinbondingagentsonistreptococcusmutansi AT monikalukomskaszymanska flowcytometryanalysisofantibacterialeffectsofuniversaldentinbondingagentsonistreptococcusmutansi |
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1716827485653434368 |