Prognostic and Predictive Value of m6A “Eraser” Related Gene Signature in Gastric Cancer
BackgroundN6-methyladenosine (m6A) RNA modification plays a critical role in gastric cancer (GC). However, the relationship between the m6A “eraser”, FTO, and ALKBH5, and the prognosis of GC still remains unclear. This study aimed to evaluate the effect of FTO and ALKBH5 on the prognosis of patients...
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doaj-0122f0ef409a46f2b30bc45fbaf4b4302021-02-26T07:07:19ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-02-011110.3389/fonc.2021.631803631803Prognostic and Predictive Value of m6A “Eraser” Related Gene Signature in Gastric CancerXin Xu0En Zhou1Jun Zheng2Chihao Zhang3Yinghua Zou4Jiayun Lin5Jiwei Yu6Department of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Cardiology, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Anesthesiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaBackgroundN6-methyladenosine (m6A) RNA modification plays a critical role in gastric cancer (GC). However, the relationship between the m6A “eraser”, FTO, and ALKBH5, and the prognosis of GC still remains unclear. This study aimed to evaluate the effect of FTO and ALKBH5 on the prognosis of patients and their potential roles in GC.Materials and MethodsA total of 738 GC samples with clinical information obtained from two independent datasets were included and divided into training set and testing set. Differential expression analysis of the m6A “eraser” related genes was performed. The LASSO Cox regression model was constructed to analyze the m6A “eraser” related risk genes. The univariate and multivariate Cox regression model were employed to identify the independent prognostic factors. Kaplan-Meier method was used for survival analysis. A nomogram model was then carried out to predict the prognosis of GC patients. Additionally, GO and KEGG analyses were conducted to identify the potential role of the m6A “eraser” related genes in GC. The relative proportion of 22 different genotypes in immune infiltrating cells was calculated by CIBERSORT algorithm.ResultsIn total, nine m6A “eraser” related risk genes and risk scores were obtained and calculated. Patients in high-risk group demonstrated significantly worse prognosis than those in low-risk group. Age, stage, and risk score were considered as independent prognostic factors. The nomogram model constructed accurately predicted the 3-year and 5-year overall survival (OS) of patients. Furthermore, m6A “eraser” might play a functional role in GC. The expression of m6A “eraser” leads to changes in tumor immune microenvironment.ConclusionsFTO and ALKBH5 showed association with the prognosis of GC. The m6A “eraser” related genes, which is considered as a reliable prognostic and predictive tool, assists in predicting the OS in GC patients.https://www.frontiersin.org/articles/10.3389/fonc.2021.631803/fullgastric cancerFTOALKBH5prognosisimmune infiltrating cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin Xu En Zhou Jun Zheng Chihao Zhang Yinghua Zou Jiayun Lin Jiwei Yu |
spellingShingle |
Xin Xu En Zhou Jun Zheng Chihao Zhang Yinghua Zou Jiayun Lin Jiwei Yu Prognostic and Predictive Value of m6A “Eraser” Related Gene Signature in Gastric Cancer Frontiers in Oncology gastric cancer FTO ALKBH5 prognosis immune infiltrating cells |
author_facet |
Xin Xu En Zhou Jun Zheng Chihao Zhang Yinghua Zou Jiayun Lin Jiwei Yu |
author_sort |
Xin Xu |
title |
Prognostic and Predictive Value of m6A “Eraser” Related Gene Signature in Gastric Cancer |
title_short |
Prognostic and Predictive Value of m6A “Eraser” Related Gene Signature in Gastric Cancer |
title_full |
Prognostic and Predictive Value of m6A “Eraser” Related Gene Signature in Gastric Cancer |
title_fullStr |
Prognostic and Predictive Value of m6A “Eraser” Related Gene Signature in Gastric Cancer |
title_full_unstemmed |
Prognostic and Predictive Value of m6A “Eraser” Related Gene Signature in Gastric Cancer |
title_sort |
prognostic and predictive value of m6a “eraser” related gene signature in gastric cancer |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2021-02-01 |
description |
BackgroundN6-methyladenosine (m6A) RNA modification plays a critical role in gastric cancer (GC). However, the relationship between the m6A “eraser”, FTO, and ALKBH5, and the prognosis of GC still remains unclear. This study aimed to evaluate the effect of FTO and ALKBH5 on the prognosis of patients and their potential roles in GC.Materials and MethodsA total of 738 GC samples with clinical information obtained from two independent datasets were included and divided into training set and testing set. Differential expression analysis of the m6A “eraser” related genes was performed. The LASSO Cox regression model was constructed to analyze the m6A “eraser” related risk genes. The univariate and multivariate Cox regression model were employed to identify the independent prognostic factors. Kaplan-Meier method was used for survival analysis. A nomogram model was then carried out to predict the prognosis of GC patients. Additionally, GO and KEGG analyses were conducted to identify the potential role of the m6A “eraser” related genes in GC. The relative proportion of 22 different genotypes in immune infiltrating cells was calculated by CIBERSORT algorithm.ResultsIn total, nine m6A “eraser” related risk genes and risk scores were obtained and calculated. Patients in high-risk group demonstrated significantly worse prognosis than those in low-risk group. Age, stage, and risk score were considered as independent prognostic factors. The nomogram model constructed accurately predicted the 3-year and 5-year overall survival (OS) of patients. Furthermore, m6A “eraser” might play a functional role in GC. The expression of m6A “eraser” leads to changes in tumor immune microenvironment.ConclusionsFTO and ALKBH5 showed association with the prognosis of GC. The m6A “eraser” related genes, which is considered as a reliable prognostic and predictive tool, assists in predicting the OS in GC patients. |
topic |
gastric cancer FTO ALKBH5 prognosis immune infiltrating cells |
url |
https://www.frontiersin.org/articles/10.3389/fonc.2021.631803/full |
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