DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation

Abstract Background Environmental pollution induces oxidative stress and apoptosis in mammalian oocytes, which can cause defects in reproduction; however, the molecular regulation of oxidative stress in oocytes is still largely unknown. In the present study, we identified that dynamin-related protei...

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Main Authors: Haolin Zhang, Zhennan Pan, Jiaqian Ju, Chunhua Xing, Xiaohan Li, Mengmeng Shan, Shaochen Sun
Format: Article
Language:English
Published: BMC 2020-08-01
Series:Journal of Animal Science and Biotechnology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s40104-020-00489-4
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spelling doaj-01287b64a86042a98efa158c88519a282020-11-25T02:58:24ZengBMCJournal of Animal Science and Biotechnology2049-18912020-08-0111111010.1186/s40104-020-00489-4DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturationHaolin Zhang0Zhennan Pan1Jiaqian Ju2Chunhua Xing3Xiaohan Li4Mengmeng Shan5Shaochen Sun6College of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityAbstract Background Environmental pollution induces oxidative stress and apoptosis in mammalian oocytes, which can cause defects in reproduction; however, the molecular regulation of oxidative stress in oocytes is still largely unknown. In the present study, we identified that dynamin-related protein 1 (DRP1) is an important molecule regulating oocyte mitochondrial function and preventing oxidative stress/apoptosis. DRP1 is a member of the dynamin GTPase superfamily localized at the mitochondrial-endoplasmic reticulum interaction site, where it regulates the fission of mitochondria and other related cellular processes. Results Our results show that DRP1 was stably expressed during different stages of porcine oocyte meiosis, and might have a potential relationship with mitochondria as it exhibited similar localization. Loss of DRP1 activity caused failed porcine oocyte maturation and cumulus cell expansion, as well as defects in polar body extrusion. Further analysis indicated that a DRP1 deficiency caused mitochondrial dysfunction and induced oxidative stress, which was confirmed by increased reactive oxygen species levels. Moreover, the incidence of early apoptosis increased as detected by positive Annexin-V signaling. Conclusions Taken together, our results indicate that DRP1 is essential for porcine oocyte maturation and that a DRP1 deficiency could induce mitochondrial dysfunction, oxidative stress, and apoptosis.http://link.springer.com/article/10.1186/s40104-020-00489-4ApoptosisMeiosisMitochondriaOocyteOxidative stress
collection DOAJ
language English
format Article
sources DOAJ
author Haolin Zhang
Zhennan Pan
Jiaqian Ju
Chunhua Xing
Xiaohan Li
Mengmeng Shan
Shaochen Sun
spellingShingle Haolin Zhang
Zhennan Pan
Jiaqian Ju
Chunhua Xing
Xiaohan Li
Mengmeng Shan
Shaochen Sun
DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation
Journal of Animal Science and Biotechnology
Apoptosis
Meiosis
Mitochondria
Oocyte
Oxidative stress
author_facet Haolin Zhang
Zhennan Pan
Jiaqian Ju
Chunhua Xing
Xiaohan Li
Mengmeng Shan
Shaochen Sun
author_sort Haolin Zhang
title DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation
title_short DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation
title_full DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation
title_fullStr DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation
title_full_unstemmed DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation
title_sort drp1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation
publisher BMC
series Journal of Animal Science and Biotechnology
issn 2049-1891
publishDate 2020-08-01
description Abstract Background Environmental pollution induces oxidative stress and apoptosis in mammalian oocytes, which can cause defects in reproduction; however, the molecular regulation of oxidative stress in oocytes is still largely unknown. In the present study, we identified that dynamin-related protein 1 (DRP1) is an important molecule regulating oocyte mitochondrial function and preventing oxidative stress/apoptosis. DRP1 is a member of the dynamin GTPase superfamily localized at the mitochondrial-endoplasmic reticulum interaction site, where it regulates the fission of mitochondria and other related cellular processes. Results Our results show that DRP1 was stably expressed during different stages of porcine oocyte meiosis, and might have a potential relationship with mitochondria as it exhibited similar localization. Loss of DRP1 activity caused failed porcine oocyte maturation and cumulus cell expansion, as well as defects in polar body extrusion. Further analysis indicated that a DRP1 deficiency caused mitochondrial dysfunction and induced oxidative stress, which was confirmed by increased reactive oxygen species levels. Moreover, the incidence of early apoptosis increased as detected by positive Annexin-V signaling. Conclusions Taken together, our results indicate that DRP1 is essential for porcine oocyte maturation and that a DRP1 deficiency could induce mitochondrial dysfunction, oxidative stress, and apoptosis.
topic Apoptosis
Meiosis
Mitochondria
Oocyte
Oxidative stress
url http://link.springer.com/article/10.1186/s40104-020-00489-4
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