Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells
We aimed to explore the effects of raloxifene (RAL) on the proliferation and apoptosis of human aortic valve interstitial cells (AVICs). Different concentrations of RAL were used to act on AVICs. MTS kit is used to test the effects of different concentrations of RAL on the proliferation of AVICs. Ce...
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Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2016/5473204 |
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doaj-0128eba6c5da43d699d5b84789cad9cd2020-11-24T22:26:25ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/54732045473204Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial CellsZhimin Fu0Bin Luo1Mingpeng Li2Bin Peng3Zheng Wang4Department of Cardiothoracic Surgery, The First Affiliated Hospital of Jinan University, Guangzhou Shi, ChinaDepartment of Cardiac Surgery, Shenzhen People’s Hospital, Shenzhen 518020, ChinaCardiovascular Department, Chenzhou First People’s Hospital of Hunan Province, Chenzhou 423000, ChinaCardiovascular Department, Chenzhou First People’s Hospital of Hunan Province, Chenzhou 423000, ChinaDepartment of Cardiothoracic Surgery, The 2nd Clinical Medical College of Jinan University, Shenzhen 518020, ChinaWe aimed to explore the effects of raloxifene (RAL) on the proliferation and apoptosis of human aortic valve interstitial cells (AVICs). Different concentrations of RAL were used to act on AVICs. MTS kit is used to test the effects of different concentrations of RAL on the proliferation of AVICs. Cell cycle and apoptosis test used flow cytometry after seven-day treatment. The relative expression levels of caspase-3 and caspase-8 are tested with RT-qPCR and Western blot. The results of MTS testing revealed that the absorbance value (OD value) of the cells in the concentration groups of 10 and 100 nmol/L RAL at a wavelength of 490 nm at five, seven, and nine days significantly decreased compared with that in the control group. Meanwhile, the results of flow cytometry of the cells collected after seven days showed that the ratio of the S stage and the cell apoptosis rate of AVICs can be significantly reduced by RAL in the concentration groups of 10 and 100 nmol/L. The mRNA and protein expressions of caspase-3 and caspase-8 were significantly decreased compared with those in the control group. This study laid the foundation for further treatment of aortic valve disease by using RAL.http://dx.doi.org/10.1155/2016/5473204 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhimin Fu Bin Luo Mingpeng Li Bin Peng Zheng Wang |
spellingShingle |
Zhimin Fu Bin Luo Mingpeng Li Bin Peng Zheng Wang Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells BioMed Research International |
author_facet |
Zhimin Fu Bin Luo Mingpeng Li Bin Peng Zheng Wang |
author_sort |
Zhimin Fu |
title |
Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells |
title_short |
Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells |
title_full |
Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells |
title_fullStr |
Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells |
title_full_unstemmed |
Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells |
title_sort |
effects of raloxifene on the proliferation and apoptosis of human aortic valve interstitial cells |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2016-01-01 |
description |
We aimed to explore the effects of raloxifene (RAL) on the proliferation and apoptosis of human aortic valve interstitial cells (AVICs). Different concentrations of RAL were used to act on AVICs. MTS kit is used to test the effects of different concentrations of RAL on the proliferation of AVICs. Cell cycle and apoptosis test used flow cytometry after seven-day treatment. The relative expression levels of caspase-3 and caspase-8 are tested with RT-qPCR and Western blot. The results of MTS testing revealed that the absorbance value (OD value) of the cells in the concentration groups of 10 and 100 nmol/L RAL at a wavelength of 490 nm at five, seven, and nine days significantly decreased compared with that in the control group. Meanwhile, the results of flow cytometry of the cells collected after seven days showed that the ratio of the S stage and the cell apoptosis rate of AVICs can be significantly reduced by RAL in the concentration groups of 10 and 100 nmol/L. The mRNA and protein expressions of caspase-3 and caspase-8 were significantly decreased compared with those in the control group. This study laid the foundation for further treatment of aortic valve disease by using RAL. |
url |
http://dx.doi.org/10.1155/2016/5473204 |
work_keys_str_mv |
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