Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285
Abstract Hydrogen gas represents a promising alternative energy source to dwindling fossil fuel reserves, as it carries the highest energy per unit mass and its combustion results in the release of water vapour as only byproduct. The facultatively anaerobic thermophile Parageobacillus thermoglucosid...
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Online Access: | https://doi.org/10.1186/s13568-019-0931-1 |
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doaj-012da0e1d3f6408faec21065f5bd45ba2020-12-27T12:10:04ZengSpringerOpenAMB Express2191-08552019-12-019111010.1186/s13568-019-0931-1Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285Teresa Mohr0Habibu Aliyu1Lars Biebinger2Roman Gödert3Alexander Hornberger4Don Cowan5Pieter de Maayer6Anke Neumann7Section II: Technical Biology, Institute of Process Engineering in Life Science, Karlsruhe Institute of TechnologySection II: Technical Biology, Institute of Process Engineering in Life Science, Karlsruhe Institute of TechnologySection II: Technical Biology, Institute of Process Engineering in Life Science, Karlsruhe Institute of TechnologySection II: Technical Biology, Institute of Process Engineering in Life Science, Karlsruhe Institute of TechnologySection II: Technical Biology, Institute of Process Engineering in Life Science, Karlsruhe Institute of TechnologyDepartment of Biochemistry, Genetics and Microbiology, Centre for Microbial Ecology and Genomics, University of PretoriaSchool of Molecular & Cell Biology, Faculty of Science, University of the WitwatersrandSection II: Technical Biology, Institute of Process Engineering in Life Science, Karlsruhe Institute of TechnologyAbstract Hydrogen gas represents a promising alternative energy source to dwindling fossil fuel reserves, as it carries the highest energy per unit mass and its combustion results in the release of water vapour as only byproduct. The facultatively anaerobic thermophile Parageobacillus thermoglucosidasius is able to produce hydrogen via the water–gas shift reaction catalyzed by a carbon monoxide dehydrogenase–hydrogenase enzyme complex. Here we have evaluated the effects of several operating parameters on hydrogen production, including different growth temperatures, pre-culture ages and inoculum sizes, as well as different pHs and concentrations of nickel and iron in the fermentation medium. All of the tested parameters were observed to have a substantive effect on both hydrogen yield and (specific) production rates. A final experiment incorporating the best scenario for each tested parameter showed a marked increase in the H2 production rate compared to each individual parameter. The optimised parameters serve as a strong basis for improved hydrogen production with a view of commercialisation of this process.https://doi.org/10.1186/s13568-019-0931-1Parageobacillus thermoglucosidasiusWater–gas shift reactionBiohydrogenProcess optimizationCO-dehydrogenase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Teresa Mohr Habibu Aliyu Lars Biebinger Roman Gödert Alexander Hornberger Don Cowan Pieter de Maayer Anke Neumann |
spellingShingle |
Teresa Mohr Habibu Aliyu Lars Biebinger Roman Gödert Alexander Hornberger Don Cowan Pieter de Maayer Anke Neumann Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285 AMB Express Parageobacillus thermoglucosidasius Water–gas shift reaction Biohydrogen Process optimization CO-dehydrogenase |
author_facet |
Teresa Mohr Habibu Aliyu Lars Biebinger Roman Gödert Alexander Hornberger Don Cowan Pieter de Maayer Anke Neumann |
author_sort |
Teresa Mohr |
title |
Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285 |
title_short |
Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285 |
title_full |
Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285 |
title_fullStr |
Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285 |
title_full_unstemmed |
Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285 |
title_sort |
effects of different operating parameters on hydrogen production by parageobacillus thermoglucosidasius dsm 6285 |
publisher |
SpringerOpen |
series |
AMB Express |
issn |
2191-0855 |
publishDate |
2019-12-01 |
description |
Abstract Hydrogen gas represents a promising alternative energy source to dwindling fossil fuel reserves, as it carries the highest energy per unit mass and its combustion results in the release of water vapour as only byproduct. The facultatively anaerobic thermophile Parageobacillus thermoglucosidasius is able to produce hydrogen via the water–gas shift reaction catalyzed by a carbon monoxide dehydrogenase–hydrogenase enzyme complex. Here we have evaluated the effects of several operating parameters on hydrogen production, including different growth temperatures, pre-culture ages and inoculum sizes, as well as different pHs and concentrations of nickel and iron in the fermentation medium. All of the tested parameters were observed to have a substantive effect on both hydrogen yield and (specific) production rates. A final experiment incorporating the best scenario for each tested parameter showed a marked increase in the H2 production rate compared to each individual parameter. The optimised parameters serve as a strong basis for improved hydrogen production with a view of commercialisation of this process. |
topic |
Parageobacillus thermoglucosidasius Water–gas shift reaction Biohydrogen Process optimization CO-dehydrogenase |
url |
https://doi.org/10.1186/s13568-019-0931-1 |
work_keys_str_mv |
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