Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform

Abstract Antibacterial surfaces coated with nanomaterials, including silver nanoparticles, are considered effective alternative antimicrobial agents that can be used instead of antibiotics and chemical agents. However, reports of the potential toxicity of these materials raise questions about the sa...

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Main Authors: Mateusz Wierzbicki, Sławomir Jaworski, Ewa Sawosz, Anna Jung, Grzegorz Gielerak, Henryk Jaremek, Witold Łojkowski, Bartosz Woźniak, Leszek Stobiński, Artur Małolepszy, André Chwalibog
Format: Article
Language:English
Published: SpringerOpen 2019-10-01
Series:Nanoscale Research Letters
Subjects:
Online Access:http://link.springer.com/article/10.1186/s11671-019-3166-9
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spelling doaj-0131d5025b294247b0de15c64e7fce3f2020-11-25T03:06:50ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2019-10-0114111110.1186/s11671-019-3166-9Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial NanoplatformMateusz Wierzbicki0Sławomir Jaworski1Ewa Sawosz2Anna Jung3Grzegorz Gielerak4Henryk Jaremek5Witold Łojkowski6Bartosz Woźniak7Leszek Stobiński8Artur Małolepszy9André Chwalibog10Institute of Biology, Department of Nanobiotechnology and Experiemntal Ecology, Warsaw University of Life SciencesInstitute of Biology, Department of Nanobiotechnology and Experiemntal Ecology, Warsaw University of Life SciencesInstitute of Biology, Department of Nanobiotechnology and Experiemntal Ecology, Warsaw University of Life SciencesMilitary Institute of MedicineMilitary Institute of MedicineBraster S.A.Institute of High Pressure Physics of the Polish Academy of SciencesInstitute of High Pressure Physics of the Polish Academy of SciencesFaculty of Chemical and Process Engineering, Warsaw University of TechnologyFaculty of Chemical and Process Engineering, Warsaw University of TechnologyDepartment of Veterinary and Animal Sciences, University of CopenhagenAbstract Antibacterial surfaces coated with nanomaterials, including silver nanoparticles, are considered effective alternative antimicrobial agents that can be used instead of antibiotics and chemical agents. However, reports of the potential toxicity of these materials raise questions about the safety of their use in biomedical applications. The objective of this research was to reduce the human cell cytotoxicity of silver nanoparticle-coated polyurethane foils by complexing silver nanoparticles with graphene oxide. The antimicrobial activity of nanoplatforms coated with silver nanoparticles, graphene oxide and the composite of silver nanoparticles and graphene oxide was assessed with Salmonella enteritidis. Cytotoxicity was analysed by an analysis of the viability and morphology of human fibroblasts, human umbilical vein endothelial cells (HUVECs) and chicken embryo chorioallantoic membrane. Additionally, the synthesis level of inflammatory proteins was examined for fibroblasts cultured on different nanoplatforms. The nanoplatform coated with the silver nanoparticles and graphene oxide composite showed strongest antibacterial properties, although nanoplatforms coated with only silver nanoparticles or graphene oxide also resulted in decreased S. enteritidis growth. Furthermore, a nanoplatform coated with silver nanoparticles and graphene oxide composite showed limited immunological stimulation and significantly reduced cytotoxicity towards fibroblasts, HUVECs and chicken embryo chorioallantoic membrane in comparison to the nanoplatform coated only with silver nanoparticles, due to the higher stability of the nanomaterials in the nanocomposite.http://link.springer.com/article/10.1186/s11671-019-3166-9Silver nanoparticlesGraphene oxideAntibacterial surfaceToxicityFibroblastsEndothelial cells
collection DOAJ
language English
format Article
sources DOAJ
author Mateusz Wierzbicki
Sławomir Jaworski
Ewa Sawosz
Anna Jung
Grzegorz Gielerak
Henryk Jaremek
Witold Łojkowski
Bartosz Woźniak
Leszek Stobiński
Artur Małolepszy
André Chwalibog
spellingShingle Mateusz Wierzbicki
Sławomir Jaworski
Ewa Sawosz
Anna Jung
Grzegorz Gielerak
Henryk Jaremek
Witold Łojkowski
Bartosz Woźniak
Leszek Stobiński
Artur Małolepszy
André Chwalibog
Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform
Nanoscale Research Letters
Silver nanoparticles
Graphene oxide
Antibacterial surface
Toxicity
Fibroblasts
Endothelial cells
author_facet Mateusz Wierzbicki
Sławomir Jaworski
Ewa Sawosz
Anna Jung
Grzegorz Gielerak
Henryk Jaremek
Witold Łojkowski
Bartosz Woźniak
Leszek Stobiński
Artur Małolepszy
André Chwalibog
author_sort Mateusz Wierzbicki
title Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform
title_short Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform
title_full Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform
title_fullStr Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform
title_full_unstemmed Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform
title_sort graphene oxide in a composite with silver nanoparticles reduces the fibroblast and endothelial cell cytotoxicity of an antibacterial nanoplatform
publisher SpringerOpen
series Nanoscale Research Letters
issn 1931-7573
1556-276X
publishDate 2019-10-01
description Abstract Antibacterial surfaces coated with nanomaterials, including silver nanoparticles, are considered effective alternative antimicrobial agents that can be used instead of antibiotics and chemical agents. However, reports of the potential toxicity of these materials raise questions about the safety of their use in biomedical applications. The objective of this research was to reduce the human cell cytotoxicity of silver nanoparticle-coated polyurethane foils by complexing silver nanoparticles with graphene oxide. The antimicrobial activity of nanoplatforms coated with silver nanoparticles, graphene oxide and the composite of silver nanoparticles and graphene oxide was assessed with Salmonella enteritidis. Cytotoxicity was analysed by an analysis of the viability and morphology of human fibroblasts, human umbilical vein endothelial cells (HUVECs) and chicken embryo chorioallantoic membrane. Additionally, the synthesis level of inflammatory proteins was examined for fibroblasts cultured on different nanoplatforms. The nanoplatform coated with the silver nanoparticles and graphene oxide composite showed strongest antibacterial properties, although nanoplatforms coated with only silver nanoparticles or graphene oxide also resulted in decreased S. enteritidis growth. Furthermore, a nanoplatform coated with silver nanoparticles and graphene oxide composite showed limited immunological stimulation and significantly reduced cytotoxicity towards fibroblasts, HUVECs and chicken embryo chorioallantoic membrane in comparison to the nanoplatform coated only with silver nanoparticles, due to the higher stability of the nanomaterials in the nanocomposite.
topic Silver nanoparticles
Graphene oxide
Antibacterial surface
Toxicity
Fibroblasts
Endothelial cells
url http://link.springer.com/article/10.1186/s11671-019-3166-9
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