Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ Mice
Colorectal cancer (CRC) is the third most common cancer in the world. Oplopanax elatus is widely used in traditional medicine. However, little is known about its pharmacological effects and bioactive compounds. We evaluated the effects of the polyyne-enriched extract from O. elatus (PEO) on the prog...
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doaj-01394d2a65504d78be6d3bdba42ca32a2020-11-24T21:27:51ZengMDPI AGMolecules1420-30492017-09-012210159310.3390/molecules22101593molecules22101593Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ MiceXin Qiao0Wei Sun1Chongzhi Wang2Li Zhang3Ping Li4Xiaodong Wen5Jie Yang6Chunsu Yuan7State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaTang Center for Herbal Medicine Research and Department of Anesthesia & Critical Care, The Pritzker School of Medicine, The University of Chicago, Chicago, IL 60601, USAState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, ChinaTang Center for Herbal Medicine Research and Department of Anesthesia & Critical Care, The Pritzker School of Medicine, The University of Chicago, Chicago, IL 60601, USAColorectal cancer (CRC) is the third most common cancer in the world. Oplopanax elatus is widely used in traditional medicine. However, little is known about its pharmacological effects and bioactive compounds. We evaluated the effects of the polyyne-enriched extract from O. elatus (PEO) on the progression of colon carcinogenesis in ApcMin/+ mice. In addition, these effects were also investigated in HCT116 and SW480 cells. After PEO oral administration (0.2% diet) for 12 weeks, PEO significantly improved body weight changes and reduced the tumor burden and tumor multiplicity compared with the untreated mice. Meanwhile, western blot and immunohistochemistry results showed PEO significantly reduced the expression of β-catenin and cyclinD1 in both small intestine and the colon tissues compared with the untreated mice. In addition, PEO treatment significant decreased the cell viability in both HCT116 and SW480 cell lines. It also decreased the levels of β-catenin, cyclinD1, c-myc and p-GSK-3β in HCT116 and SW480 cells at 25 μM. These results indicate that PEO may have potential value in prevention of colon cancer by down-regulating Wnt-related protein.https://www.mdpi.com/1420-3049/22/10/1593PEOcolorectal cancerWnt/β-catenin signaling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin Qiao Wei Sun Chongzhi Wang Li Zhang Ping Li Xiaodong Wen Jie Yang Chunsu Yuan |
spellingShingle |
Xin Qiao Wei Sun Chongzhi Wang Li Zhang Ping Li Xiaodong Wen Jie Yang Chunsu Yuan Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ Mice Molecules PEO colorectal cancer Wnt/β-catenin signaling |
author_facet |
Xin Qiao Wei Sun Chongzhi Wang Li Zhang Ping Li Xiaodong Wen Jie Yang Chunsu Yuan |
author_sort |
Xin Qiao |
title |
Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ Mice |
title_short |
Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ Mice |
title_full |
Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ Mice |
title_fullStr |
Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ Mice |
title_full_unstemmed |
Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ Mice |
title_sort |
polyyne-enriched extract from oplopanax elatus significantly ameliorates the progression of colon carcinogenesis in apcmin/+ mice |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2017-09-01 |
description |
Colorectal cancer (CRC) is the third most common cancer in the world. Oplopanax elatus is widely used in traditional medicine. However, little is known about its pharmacological effects and bioactive compounds. We evaluated the effects of the polyyne-enriched extract from O. elatus (PEO) on the progression of colon carcinogenesis in ApcMin/+ mice. In addition, these effects were also investigated in HCT116 and SW480 cells. After PEO oral administration (0.2% diet) for 12 weeks, PEO significantly improved body weight changes and reduced the tumor burden and tumor multiplicity compared with the untreated mice. Meanwhile, western blot and immunohistochemistry results showed PEO significantly reduced the expression of β-catenin and cyclinD1 in both small intestine and the colon tissues compared with the untreated mice. In addition, PEO treatment significant decreased the cell viability in both HCT116 and SW480 cell lines. It also decreased the levels of β-catenin, cyclinD1, c-myc and p-GSK-3β in HCT116 and SW480 cells at 25 μM. These results indicate that PEO may have potential value in prevention of colon cancer by down-regulating Wnt-related protein. |
topic |
PEO colorectal cancer Wnt/β-catenin signaling |
url |
https://www.mdpi.com/1420-3049/22/10/1593 |
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