Effect of transplantation of muscle tissue in rats from the same litter on total number of flavins and FAD

• Main Authors: S. N. Kobylnik, I. L. Vovchuk, O. O. Dosenko, S. N. Kozishkurt, D. V. Morosova, S. A. Petrov
• Format: Article
• Language: English
• Published: Oles Honchar Dnipropetrovsk National University 2015-01-01
• Series: Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Medicina
• Subjects: transplantation; riboflavin; FMN; FAD
• Online Access: http://medicine.dp.ua/index.php/med/article/view/240

Riboflavin is a member of redox enzymes involved in fatty acid oxidation and energy generation. Important role of this vitamin is in reproductive function. Exchange of transformation of riboflavin in animal tissues and cells of microorganisms include reactions that lead to synthesis and subsequent collapse of FMN and FAD. It is involved in enhancing antitumor activity of many anticancer drugs, as well as activation of the immune system to kill tumor cells. Issues of transport of riboflavin and its derivatives in animals have been studied enough. Investigations of changes of the balance of riboflavin and its metabolites in muscular tissues before transplantation in rats from one litter and at operation without replanting were conducted, based on the Udenfriend method of flavin determination. Transplantation in the experiment was carried out on white non-linear male rats weighing 180–300 g. Animals were taken out of the experiment by passing electric current through the medulla. Belly muscular tissue was taken from donor rats of the same litter, and that tissue was sewn to homological muscular tissue of the recipient. The same procedure was carried out with femoral muscular tissue. In the course of operation without replanting the same manipulations have been made except for transplantation stage (for determination of the effect of surgical intervention). Tissue not subject to any surgical intervention served as a control. Parameters of the study were measured on the first, third and seventh days after transplantation. Transplantation of muscular tissue caused no changes in total flavin amount. Content of RF + FMN after transplantation of muscular tissue in rats of the same litter decreased in femoral muscular tissue of the recipient. Transplantation of muscular tissues in rats from the same litter lead to increase in FAD amount in femoral muscular tissue of the donor and recipient on the third day of the experiment. Transplantation of femoral muscular tissue lead to acceleration of FAD synthesis from riboflavin and FMN.