Screening for Early Gastric Cancer Using a Noninvasive Urine Metabolomics Approach
The early detection of gastric cancer (GC) could decrease its incidence and mortality. However, there are currently no accurate noninvasive markers for GC screening. Therefore, we developed a noninvasive diagnostic approach, employing urine nuclear magnetic resonance (NMR) metabolomics, to discover...
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doaj-01417bb7136e4865af83523c4155cc402020-11-25T03:55:19ZengMDPI AGCancers2072-66942020-10-01122904290410.3390/cancers12102904Screening for Early Gastric Cancer Using a Noninvasive Urine Metabolomics ApproachHyuk Nam Kwon0Hyuk Lee1Ji Won Park2Young-Ho Kim3Sunghyouk Park4Jae J. Kim5College of Pharmacy, Natural Product Research Institute, Seoul National University, Seoul 08826, KoreaDepartment of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaDepartment of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaDepartment of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaCollege of Pharmacy, Natural Product Research Institute, Seoul National University, Seoul 08826, KoreaDepartment of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaThe early detection of gastric cancer (GC) could decrease its incidence and mortality. However, there are currently no accurate noninvasive markers for GC screening. Therefore, we developed a noninvasive diagnostic approach, employing urine nuclear magnetic resonance (NMR) metabolomics, to discover putative metabolic markers associated with GC. Changes in urine metabolite levels during oncogenesis were evaluated using samples from 103 patients with GC and 100 age- and sex-matched healthy controls. Approximately 70% of the patients with GC (<i>n</i> = 69) had stage I GC, with the majority (<i>n</i> = 56) having intramucosal cancer. A multivariate statistical analysis of the urine NMR data well discriminated between the patient and control groups and revealed nine metabolites, including alanine, citrate, creatine, creatinine, glycerol, hippurate, phenylalanine, taurine, and 3-hydroxybutyrate, that contributed to the difference. A diagnostic performance test with a separate validation set exhibited a sensitivity and specificity of more than 90%, even with the intramucosal cancer samples only. In conclusion, the NMR-based urine metabolomics approach may have potential as a convenient screening method for the early detection of GC and may facilitate consequent endoscopic examination through risk stratification.https://www.mdpi.com/2072-6694/12/10/2904gastric cancerscreeningmetabolomicsurine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyuk Nam Kwon Hyuk Lee Ji Won Park Young-Ho Kim Sunghyouk Park Jae J. Kim |
spellingShingle |
Hyuk Nam Kwon Hyuk Lee Ji Won Park Young-Ho Kim Sunghyouk Park Jae J. Kim Screening for Early Gastric Cancer Using a Noninvasive Urine Metabolomics Approach Cancers gastric cancer screening metabolomics urine |
author_facet |
Hyuk Nam Kwon Hyuk Lee Ji Won Park Young-Ho Kim Sunghyouk Park Jae J. Kim |
author_sort |
Hyuk Nam Kwon |
title |
Screening for Early Gastric Cancer Using a Noninvasive Urine Metabolomics Approach |
title_short |
Screening for Early Gastric Cancer Using a Noninvasive Urine Metabolomics Approach |
title_full |
Screening for Early Gastric Cancer Using a Noninvasive Urine Metabolomics Approach |
title_fullStr |
Screening for Early Gastric Cancer Using a Noninvasive Urine Metabolomics Approach |
title_full_unstemmed |
Screening for Early Gastric Cancer Using a Noninvasive Urine Metabolomics Approach |
title_sort |
screening for early gastric cancer using a noninvasive urine metabolomics approach |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2020-10-01 |
description |
The early detection of gastric cancer (GC) could decrease its incidence and mortality. However, there are currently no accurate noninvasive markers for GC screening. Therefore, we developed a noninvasive diagnostic approach, employing urine nuclear magnetic resonance (NMR) metabolomics, to discover putative metabolic markers associated with GC. Changes in urine metabolite levels during oncogenesis were evaluated using samples from 103 patients with GC and 100 age- and sex-matched healthy controls. Approximately 70% of the patients with GC (<i>n</i> = 69) had stage I GC, with the majority (<i>n</i> = 56) having intramucosal cancer. A multivariate statistical analysis of the urine NMR data well discriminated between the patient and control groups and revealed nine metabolites, including alanine, citrate, creatine, creatinine, glycerol, hippurate, phenylalanine, taurine, and 3-hydroxybutyrate, that contributed to the difference. A diagnostic performance test with a separate validation set exhibited a sensitivity and specificity of more than 90%, even with the intramucosal cancer samples only. In conclusion, the NMR-based urine metabolomics approach may have potential as a convenient screening method for the early detection of GC and may facilitate consequent endoscopic examination through risk stratification. |
topic |
gastric cancer screening metabolomics urine |
url |
https://www.mdpi.com/2072-6694/12/10/2904 |
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