Disinfection for the IN VITRO establishment of onion Ocañera Allium Cepa L

• Main Authors: Jose Arnoldo Granadillo Cuello, Elibardo Pacheco Carrascal
• Format: Article
• Language: English
• Published: Universidad Francisco de Paula Santander Ocaña 2015-01-01
• Series: Revista Ingenio
• Subjects: micropropagation; establishment; in vitro; bacteriosis; virosis; fusariums
• Online Access: https://revistas.ufps.edu.co/index.php/ingenio/article/view/2041/1994

The Ocañera Onion Allium cepa L. is characterized by its asexual planting (bulbs), unlike varieties sown from sexual seed. In this type of sowing, phytosanitary problems are promoted, causing growers to apply pesticides periodically, raising production costs, significantly reducing crop yields and generating economic crisis in the region. Propagation methods have been studied by in-vitro tissue culture, which allows the regeneration of plants of a desirable genotype under aseptic conditions to rid them of diseases. The objective of this research was to standardize a disinfection protocol to establish in-vitro seedlings of ocañera onion, lowering the levels of contamination with bacteria, viruses and fungi. The experimental design was completely randomized, with a variety of Red creole onion and four disinfection treatments. The meristems were inoculated in establishment culture medium. The variable analyzed was the number of contaminated explants at 4, 8 and 12 days after establishment. The lowest contamination index was 12% in treatment D, which was maintained until 12 days after establishment.