DETECTION OF LASALOCID RESIDUES IN THE TISSUES OF BROILER CHICKENS BY A NEW SCREENING TEST TOTAL ANTIBIOTICS

• Main Authors: Martin Levkut, ml., Ivona Kožárová, Jana Šimková, Mária Mártonová, Ján Mačanga
• Format: Article
• Language: English
• Published: HACCP Consulting 2011-04-01
• Series: Potravinarstvo
• Subjects: lasalocid, residue, tissues of broiler chickens, screening, Total antibiotics
• Online Access: http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/140

<p class="MsoNormal" style="text-justify: inter-ideograph; margin: 0cm 0cm 0pt; line-height: 150%; text-align: justify; mso-layout-grid-align: none;"><span style="font-size: small;"><span style="font-family: Times New Roman;"><span style="mso-ansi-language: EN-GB;" lang="EN-GB">The aim of the present study was to evaluate the microbial growth inhibition test Total antibiotics for the screening of lasalocid residues in the tissues of broiler chickens after its oral administration in medicated feed. The residues were investigated throughout the 5-day withdrawal period /WP/) and also on day 6 representing the first day following the WP. </span><span style="mso-ansi-language: EN-GB; mso-fareast-font-family: Batang; mso-fareast-language: KO;" lang="EN-GB">All broiler chicken tissues were positive for lasalocid. The breast muscle was positive (the presence of residues at/above the detection limit /LOD/ of method) up to day 1 of the WP, the thigh muscle, gizzard, heart, skin and fat up to day 3 of the WP and the liver and kidneys</span><span style="mso-ansi-language: EN-GB;" lang="EN-GB"> up to day 4 of the WP. When evaluating the dubious results (</span><span style="mso-ansi-language: EN-GB; mso-fareast-font-family: Batang; mso-fareast-language: KO;" lang="EN-GB">the presence of residues just below the LOD of method), the breast muscle was suspect positive up to day 3 of the WP and the gizzard, skin and fat up to day 4 of the WP. </span><span style="mso-ansi-language: EN-GB;" lang="EN-GB">No positive or dubious results were detected on day 5 of the WP. </span><span style="mso-ansi-language: EN-GB; mso-fareast-font-family: Batang; mso-fareast-language: KO;" lang="EN-GB">The LOD of </span><em style="mso-bidi-font-style: normal;"><span style="mso-ansi-language: EN-GB;" lang="EN-GB">Bacillus stearothermophilus </span></em><span style="mso-ansi-language: EN-GB; mso-bidi-font-style: italic;" lang="EN-GB">var. <em>c</em></span><em style="mso-bidi-font-style: normal;"><span style="mso-ansi-language: EN-GB;" lang="EN-GB">alidolactis</span></em><span style="mso-ansi-language: EN-GB; mso-bidi-font-style: italic;" lang="EN-GB"> </span><span style="mso-ansi-language: EN-GB; mso-fareast-font-family: Batang; mso-fareast-language: KO;" lang="EN-GB">for maduramycin was </span><span style="mso-ansi-language: EN-GB;" lang="EN-GB">500 &micro;g.l^{<span style="text-transform: uppercase;">-1</span>}<span style="text-transform: uppercase;">.<br /><br /><span style="color: #111111; font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 9px; line-height: normal; text-transform: none;"><strong>doi:10.5219/140</strong></span></span></span></span></span></p>