PCR-RFLP diagnostic method for identifying <I>Globodera</I> species in Slovenia
Species identification within the genus Globodera is based on the morphological and morphometrical characters of the cysts and second stage juveniles, and these are included in the majority of identification keys. Morphometrical methods are fast and can be applied to most of samples but they demand...
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Firenze University Press
2011-01-01
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doaj-019d11d53a794f2898b61588e6b947b72020-11-25T03:21:41ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952011-01-0149310.14601/Phytopathol_Mediterr-86528671PCR-RFLP diagnostic method for identifying <I>Globodera</I> species in SloveniaSasa ŠIRCA0Barbara GERIC STARE1Polona STRAJNAR2Gregor UREK3Agricultural Institute of SloveniaAgricultural Institute of SloveniaAgricultural Institute of SloveniaAgricultural Institute of SloveniaSpecies identification within the genus Globodera is based on the morphological and morphometrical characters of the cysts and second stage juveniles, and these are included in the majority of identification keys. Morphometrical methods are fast and can be applied to most of samples but they demand a trained and experienced specialist. Furthermore, some morphometrical characters may overlap between populations and beetwen species, leading to inaccurate identification. To confirm and complement the morphometrical identification of Globodera species molecular methods have been developed. Sequences of the internal transcribed spacer regions ITS1 and ITS2 of the rDNA gene cluster proved to be useful for identifying nematode species identification. A PCR-RFLP molecular method was used to identify Globodera rostochiensis, G. pallida, G. tabacum and G. achilleae. Globodera rostochiensis, G. pallida, G. tabacum and G. achilleae can be distinguished with PCR-RFLP analysis of the rDNA ITS fragment using five restriction enzymes. The RFLP patterns of G. rostochiensis, G. tabacum and G. achilleae were species-specific, while those of G. pallida varied. South American populations of G. pallida differed from other populations as their RFLP patterns were demonstrated to be distinct by in silico restriction of the ITS sequences deposited at NCBI. https://oajournals.fupress.net/index.php/pm/article/view/5379 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sasa ŠIRCA Barbara GERIC STARE Polona STRAJNAR Gregor UREK |
spellingShingle |
Sasa ŠIRCA Barbara GERIC STARE Polona STRAJNAR Gregor UREK PCR-RFLP diagnostic method for identifying <I>Globodera</I> species in Slovenia Phytopathologia Mediterranea |
author_facet |
Sasa ŠIRCA Barbara GERIC STARE Polona STRAJNAR Gregor UREK |
author_sort |
Sasa ŠIRCA |
title |
PCR-RFLP diagnostic method for identifying <I>Globodera</I> species in Slovenia |
title_short |
PCR-RFLP diagnostic method for identifying <I>Globodera</I> species in Slovenia |
title_full |
PCR-RFLP diagnostic method for identifying <I>Globodera</I> species in Slovenia |
title_fullStr |
PCR-RFLP diagnostic method for identifying <I>Globodera</I> species in Slovenia |
title_full_unstemmed |
PCR-RFLP diagnostic method for identifying <I>Globodera</I> species in Slovenia |
title_sort |
pcr-rflp diagnostic method for identifying <i>globodera</i> species in slovenia |
publisher |
Firenze University Press |
series |
Phytopathologia Mediterranea |
issn |
0031-9465 1593-2095 |
publishDate |
2011-01-01 |
description |
Species identification within the genus Globodera is based on the morphological and morphometrical characters of the cysts and second stage juveniles, and these are included in the majority of identification keys. Morphometrical methods are fast and can be applied to most of samples but they demand a trained and experienced specialist. Furthermore, some morphometrical characters may overlap between populations and beetwen species, leading to inaccurate identification. To confirm and complement the morphometrical identification of Globodera species molecular methods have been developed. Sequences of the internal transcribed spacer regions ITS1 and ITS2 of the rDNA gene cluster proved to be useful for identifying nematode species identification. A PCR-RFLP molecular method was used to identify Globodera rostochiensis, G. pallida, G. tabacum and G. achilleae. Globodera rostochiensis, G. pallida, G. tabacum and G. achilleae can be distinguished with PCR-RFLP analysis of the rDNA ITS fragment using five restriction enzymes. The RFLP patterns of G. rostochiensis, G. tabacum and G. achilleae were species-specific, while those of G. pallida varied. South American populations of G. pallida differed from other populations as their RFLP patterns were demonstrated to be distinct by in silico restriction of the ITS sequences deposited at NCBI.
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url |
https://oajournals.fupress.net/index.php/pm/article/view/5379 |
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