Dual extraction of mRNA and lipids from a single biological sample
Abstract The extraction of RNA and lipids from a large number of biological samples is time-consuming and costly with steps required for both transcriptomic and lipidomic approaches. Most protocols rely on independent extraction of nucleic acids and lipids from a single sample, thereby increasing th...
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doaj-01a52063dceb482f86e9826f98b7cb9c2020-12-08T03:55:38ZengNature Publishing GroupScientific Reports2045-23222018-05-018111110.1038/s41598-018-25332-9Dual extraction of mRNA and lipids from a single biological sampleNormand Podechard0Simon Ducheix1Arnaud Polizzi2Frédéric Lasserre3Alexandra Montagner4Vincent Legagneux5Edwin Fouché6Fabrice Saez7Jean-Marc Lobaccaro8Laila Lakhal9Sandrine Ellero-Simatos10Pascal. G. Martin11Nicolas Loiseau12Justine Bertrand-Michel13Hervé Guillou14INRA UMR1331, ToxAlim, University of ToulouseINRA UMR1331, ToxAlim, University of ToulouseINRA UMR1331, ToxAlim, University of ToulouseINRA UMR1331, ToxAlim, University of ToulouseINRA UMR1331, ToxAlim, University of ToulouseIrset - Inserm UMR 1085, UFR des Sciences Pharmaceutiques et Biologiques, 35043 Rennes Cédex, France / Biosit SFR UMS CNRS 3480, Université de Rennes 1INRA UMR1331, ToxAlim, University of ToulouseUniversité Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28, place Henri Dunant, BP38Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28, place Henri Dunant, BP38INRA UMR1331, ToxAlim, University of ToulouseINRA UMR1331, ToxAlim, University of ToulouseINRA UMR1331, ToxAlim, University of ToulouseINRA UMR1331, ToxAlim, University of ToulouseInstitut National de la Santé et de la Recherche Médicale (INSERM), UMR1048, Institute of Metabolic and Cardiovascular DiseasesINRA UMR1331, ToxAlim, University of ToulouseAbstract The extraction of RNA and lipids from a large number of biological samples is time-consuming and costly with steps required for both transcriptomic and lipidomic approaches. Most protocols rely on independent extraction of nucleic acids and lipids from a single sample, thereby increasing the need for biological material and inducing variability in data analysis. We investigated whether it is possible to use a standard RNA extraction procedure to analyze not only RNA levels, but also lipids in a single liver sample. We show that the organic phase obtained when using standard reagents for RNA extraction can be used to analyze lipids, including neutral lipids and fatty acids, by gas chromatography. We applied this technique to an analysis of lipids and the associated gene expression pattern in mice with hepatic steatosis induced by pharmacological activation of nuclear receptor LXR.https://doi.org/10.1038/s41598-018-25332-9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Normand Podechard Simon Ducheix Arnaud Polizzi Frédéric Lasserre Alexandra Montagner Vincent Legagneux Edwin Fouché Fabrice Saez Jean-Marc Lobaccaro Laila Lakhal Sandrine Ellero-Simatos Pascal. G. Martin Nicolas Loiseau Justine Bertrand-Michel Hervé Guillou |
spellingShingle |
Normand Podechard Simon Ducheix Arnaud Polizzi Frédéric Lasserre Alexandra Montagner Vincent Legagneux Edwin Fouché Fabrice Saez Jean-Marc Lobaccaro Laila Lakhal Sandrine Ellero-Simatos Pascal. G. Martin Nicolas Loiseau Justine Bertrand-Michel Hervé Guillou Dual extraction of mRNA and lipids from a single biological sample Scientific Reports |
author_facet |
Normand Podechard Simon Ducheix Arnaud Polizzi Frédéric Lasserre Alexandra Montagner Vincent Legagneux Edwin Fouché Fabrice Saez Jean-Marc Lobaccaro Laila Lakhal Sandrine Ellero-Simatos Pascal. G. Martin Nicolas Loiseau Justine Bertrand-Michel Hervé Guillou |
author_sort |
Normand Podechard |
title |
Dual extraction of mRNA and lipids from a single biological sample |
title_short |
Dual extraction of mRNA and lipids from a single biological sample |
title_full |
Dual extraction of mRNA and lipids from a single biological sample |
title_fullStr |
Dual extraction of mRNA and lipids from a single biological sample |
title_full_unstemmed |
Dual extraction of mRNA and lipids from a single biological sample |
title_sort |
dual extraction of mrna and lipids from a single biological sample |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-05-01 |
description |
Abstract The extraction of RNA and lipids from a large number of biological samples is time-consuming and costly with steps required for both transcriptomic and lipidomic approaches. Most protocols rely on independent extraction of nucleic acids and lipids from a single sample, thereby increasing the need for biological material and inducing variability in data analysis. We investigated whether it is possible to use a standard RNA extraction procedure to analyze not only RNA levels, but also lipids in a single liver sample. We show that the organic phase obtained when using standard reagents for RNA extraction can be used to analyze lipids, including neutral lipids and fatty acids, by gas chromatography. We applied this technique to an analysis of lipids and the associated gene expression pattern in mice with hepatic steatosis induced by pharmacological activation of nuclear receptor LXR. |
url |
https://doi.org/10.1038/s41598-018-25332-9 |
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