A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix

A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix

A simple and promising H2O2 biosensor has been developed by successfulentrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linkedwith formaldehyde. The large microscopic surface area and porous morphology of thegelatine matrix lead to high enzyme loading and the enzyme ent...

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Bibliographic Details
Main Authors: Jun-Jie Zhu, Yan-Li Wei, Nan Li, Hui Yao
Format: Article
Language:English
Published: MDPI AG 2005-05-01
Series:Sensors
Subjects:
H2O2 biosensor
horseradish peroxidase
gelatine matrix.
Online Access:http://www.mdpi.com/1424-8220/5/4/277/
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spelling doaj-01ae4d1ad0b64b3ea2c17d7fb883748b2020-11-24T20:48:00ZengMDPI AGSensors1424-82202005-05-015427728310.3390/s5040277A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network MatrixJun-Jie ZhuYan-Li WeiNan LiHui YaoA simple and promising H2O2 biosensor has been developed by successfulentrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linkedwith formaldehyde. The large microscopic surface area and porous morphology of thegelatine matrix lead to high enzyme loading and the enzyme entrapped in this matrix canretain its bioactivity. This biosensor exhibited a fast amperometric response to hydrogenperoxide (H2O2). The linear range for H2O2 determination was from 2.5×10-5 to2.5×10-3 M, with a detection limit of 2.0×10-6 M based on S / N = 3. This biosensorpossessed very good reproducibility.http://www.mdpi.com/1424-8220/5/4/277/H2O2 biosensorhorseradish peroxidasegelatine matrix.
collection DOAJ
language English
format Article
sources DOAJ
author Jun-Jie Zhu
Yan-Li Wei
Nan Li
Hui Yao
spellingShingle Jun-Jie Zhu
Yan-Li Wei
Nan Li
Hui Yao
A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix
Sensors
H2O2 biosensor
horseradish peroxidase
gelatine matrix.
author_facet Jun-Jie Zhu
Yan-Li Wei
Nan Li
Hui Yao
author_sort Jun-Jie Zhu
title A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix
title_short A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix
title_full A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix
title_fullStr A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix
title_full_unstemmed A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix
title_sort h2o2 biosensor based on immobilization of horseradishperoxidase in a gelatine network matrix
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2005-05-01
description A simple and promising H2O2 biosensor has been developed by successfulentrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linkedwith formaldehyde. The large microscopic surface area and porous morphology of thegelatine matrix lead to high enzyme loading and the enzyme entrapped in this matrix canretain its bioactivity. This biosensor exhibited a fast amperometric response to hydrogenperoxide (H2O2). The linear range for H2O2 determination was from 2.5×10-5 to2.5×10-3 M, with a detection limit of 2.0×10-6 M based on S / N = 3. This biosensorpossessed very good reproducibility.
topic H2O2 biosensor
horseradish peroxidase
gelatine matrix.
url http://www.mdpi.com/1424-8220/5/4/277/
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