A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix
A simple and promising H2O2 biosensor has been developed by successfulentrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linkedwith formaldehyde. The large microscopic surface area and porous morphology of thegelatine matrix lead to high enzyme loading and the enzyme ent...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2005-05-01
|
Series: | Sensors |
Subjects: | |
Online Access: | http://www.mdpi.com/1424-8220/5/4/277/ |
id |
doaj-01ae4d1ad0b64b3ea2c17d7fb883748b |
---|---|
record_format |
Article |
spelling |
doaj-01ae4d1ad0b64b3ea2c17d7fb883748b2020-11-24T20:48:00ZengMDPI AGSensors1424-82202005-05-015427728310.3390/s5040277A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network MatrixJun-Jie ZhuYan-Li WeiNan LiHui YaoA simple and promising H2O2 biosensor has been developed by successfulentrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linkedwith formaldehyde. The large microscopic surface area and porous morphology of thegelatine matrix lead to high enzyme loading and the enzyme entrapped in this matrix canretain its bioactivity. This biosensor exhibited a fast amperometric response to hydrogenperoxide (H2O2). The linear range for H2O2 determination was from 2.5×10-5 to2.5×10-3 M, with a detection limit of 2.0×10-6 M based on S / N = 3. This biosensorpossessed very good reproducibility.http://www.mdpi.com/1424-8220/5/4/277/H2O2 biosensorhorseradish peroxidasegelatine matrix. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun-Jie Zhu Yan-Li Wei Nan Li Hui Yao |
spellingShingle |
Jun-Jie Zhu Yan-Li Wei Nan Li Hui Yao A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix Sensors H2O2 biosensor horseradish peroxidase gelatine matrix. |
author_facet |
Jun-Jie Zhu Yan-Li Wei Nan Li Hui Yao |
author_sort |
Jun-Jie Zhu |
title |
A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix |
title_short |
A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix |
title_full |
A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix |
title_fullStr |
A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix |
title_full_unstemmed |
A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix |
title_sort |
h2o2 biosensor based on immobilization of horseradishperoxidase in a gelatine network matrix |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2005-05-01 |
description |
A simple and promising H2O2 biosensor has been developed by successfulentrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linkedwith formaldehyde. The large microscopic surface area and porous morphology of thegelatine matrix lead to high enzyme loading and the enzyme entrapped in this matrix canretain its bioactivity. This biosensor exhibited a fast amperometric response to hydrogenperoxide (H2O2). The linear range for H2O2 determination was from 2.5×10-5 to2.5×10-3 M, with a detection limit of 2.0×10-6 M based on S / N = 3. This biosensorpossessed very good reproducibility. |
topic |
H2O2 biosensor horseradish peroxidase gelatine matrix. |
url |
http://www.mdpi.com/1424-8220/5/4/277/ |
work_keys_str_mv |
AT junjiezhu ah2o2biosensorbasedonimmobilizationofhorseradishperoxidaseinagelatinenetworkmatrix AT yanliwei ah2o2biosensorbasedonimmobilizationofhorseradishperoxidaseinagelatinenetworkmatrix AT nanli ah2o2biosensorbasedonimmobilizationofhorseradishperoxidaseinagelatinenetworkmatrix AT huiyao ah2o2biosensorbasedonimmobilizationofhorseradishperoxidaseinagelatinenetworkmatrix AT junjiezhu h2o2biosensorbasedonimmobilizationofhorseradishperoxidaseinagelatinenetworkmatrix AT yanliwei h2o2biosensorbasedonimmobilizationofhorseradishperoxidaseinagelatinenetworkmatrix AT nanli h2o2biosensorbasedonimmobilizationofhorseradishperoxidaseinagelatinenetworkmatrix AT huiyao h2o2biosensorbasedonimmobilizationofhorseradishperoxidaseinagelatinenetworkmatrix |
_version_ |
1716809269368586240 |