LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer
Abstract Background MCM3AP-AS1 is a recently characterized lncRNA playing an oncogenic role in several cancers. However, its role in lung cancer remains unknown. Here, we aimed to explore the functions of MCM3AP-AS1 in small cell lung cancer (SCLC) and the possible underlying mechanisms. Methods MCM...
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doaj-01b54ec498d74bdab2d8329daf638f272021-07-18T11:36:57ZengBMCBMC Cancer1471-24072021-07-012111810.1186/s12885-021-08365-8LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancerHua Luo0Yukun Zhang1Guangmei Qin2Bing Jiang3Lili Miao4Department of Respiratory and Critical Care Medicine, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Respiratory and Critical Care Medicine, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Respiratory and Critical Care Medicine, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Respiratory and Critical Care Medicine, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Respiratory and Critical Care Medicine, Yongchuan Hospital of Chongqing Medical UniversityAbstract Background MCM3AP-AS1 is a recently characterized lncRNA playing an oncogenic role in several cancers. However, its role in lung cancer remains unknown. Here, we aimed to explore the functions of MCM3AP-AS1 in small cell lung cancer (SCLC) and the possible underlying mechanisms. Methods MCM3AP-AS1 and ROCK1 levels in SCLC patients were analyzed by qPCR. RNA pull-down and luciferase assays were performed to analyze the interaction between MCM3AP-AS1 and miR-148a. ROCK1 mRNA and protein levels were detected by qPCR and Western blot, respectively. Cell invasion and migration were analyzed by Transwell assays. Results MCM3AP-AS1 was upregulated in patients with SCLC, and a high MCM3AP-AS1 level was accompanied by a low survival rate. The binding of MCM3AP-AS1 to miR-148a predicted by bioinformatics analysis was verified by RNA pull-down and luciferase assays. However, MCM3AP-AS1 and miR-148a did not affect each other’s expression. ROCK1 was upregulated in SCLC tissues and positively correlated with MCM3AP-AS1. In SCLC cells, MCM3AP-AS1 overexpression increased ROCK1 and promoted cancer cell invasion and migration, while miR-148a overexpression showed the opposite effects and attenuated the effects of MCM3AP-AS1 overexpression on ROCK1 expression and cell behaviors. Conclusions MCM3AP-AS1 sponges miR-148a, thereby increasing SCLC cell invasion and migration via upregulating ROCK1 expression.https://doi.org/10.1186/s12885-021-08365-8MCM3AP-AS1Small cell lung cancerSurvivalmiR-148aROCK1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hua Luo Yukun Zhang Guangmei Qin Bing Jiang Lili Miao |
spellingShingle |
Hua Luo Yukun Zhang Guangmei Qin Bing Jiang Lili Miao LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer BMC Cancer MCM3AP-AS1 Small cell lung cancer Survival miR-148a ROCK1 |
author_facet |
Hua Luo Yukun Zhang Guangmei Qin Bing Jiang Lili Miao |
author_sort |
Hua Luo |
title |
LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer |
title_short |
LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer |
title_full |
LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer |
title_fullStr |
LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer |
title_full_unstemmed |
LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer |
title_sort |
lncrna mcm3ap-as1 sponges mir-148a to enhance cell invasion and migration in small cell lung cancer |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2021-07-01 |
description |
Abstract Background MCM3AP-AS1 is a recently characterized lncRNA playing an oncogenic role in several cancers. However, its role in lung cancer remains unknown. Here, we aimed to explore the functions of MCM3AP-AS1 in small cell lung cancer (SCLC) and the possible underlying mechanisms. Methods MCM3AP-AS1 and ROCK1 levels in SCLC patients were analyzed by qPCR. RNA pull-down and luciferase assays were performed to analyze the interaction between MCM3AP-AS1 and miR-148a. ROCK1 mRNA and protein levels were detected by qPCR and Western blot, respectively. Cell invasion and migration were analyzed by Transwell assays. Results MCM3AP-AS1 was upregulated in patients with SCLC, and a high MCM3AP-AS1 level was accompanied by a low survival rate. The binding of MCM3AP-AS1 to miR-148a predicted by bioinformatics analysis was verified by RNA pull-down and luciferase assays. However, MCM3AP-AS1 and miR-148a did not affect each other’s expression. ROCK1 was upregulated in SCLC tissues and positively correlated with MCM3AP-AS1. In SCLC cells, MCM3AP-AS1 overexpression increased ROCK1 and promoted cancer cell invasion and migration, while miR-148a overexpression showed the opposite effects and attenuated the effects of MCM3AP-AS1 overexpression on ROCK1 expression and cell behaviors. Conclusions MCM3AP-AS1 sponges miR-148a, thereby increasing SCLC cell invasion and migration via upregulating ROCK1 expression. |
topic |
MCM3AP-AS1 Small cell lung cancer Survival miR-148a ROCK1 |
url |
https://doi.org/10.1186/s12885-021-08365-8 |
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