Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thaliana
Volatile allyl isothiocyanate (AITC) derives from the biodegradation of the glucosinolate sinigrin and has been associated with growth inhibition in several plants, including the model plant Arabidopsis thaliana. However, the underlying cellular mechanisms of this feature remain scarcely investigate...
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doaj-01c6f6a9d20248399f529a2c62c77c572020-11-24T22:18:12ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-12-011612291342914710.3390/ijms161226154ijms161226154Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thalianaBjørnar Sporsheim0Anders Øverby1Atle Magnar Bones2Department of Biology, the Norwegian University of Science and Technology, Høgskoleringen 5, N-7491 Trondheim, NorwayDepartment of Biology, the Norwegian University of Science and Technology, Høgskoleringen 5, N-7491 Trondheim, NorwayDepartment of Biology, the Norwegian University of Science and Technology, Høgskoleringen 5, N-7491 Trondheim, NorwayVolatile allyl isothiocyanate (AITC) derives from the biodegradation of the glucosinolate sinigrin and has been associated with growth inhibition in several plants, including the model plant Arabidopsis thaliana. However, the underlying cellular mechanisms of this feature remain scarcely investigated in plants. In this study, we present evidence of an AITC-induced inhibition of actin-dependent intracellular transport in A. thaliana. A transgenic line of A. thaliana expressing yellow fluorescent protein (YFP)-tagged actin filaments was used to show attenuation of actin filament movement by AITC. This appeared gradually in a time- and dose-dependent manner and resulted in actin filaments appearing close to static. Further, we employed four transgenic lines with YFP-fusion proteins labeling the Golgi apparatus, endoplasmic reticulum (ER), vacuoles and peroxisomes to demonstrate an AITC-induced inhibition of actin-dependent intracellular transport of or, in these structures, consistent with the decline in actin filament movement. Furthermore, the morphologies of actin filaments, ER and vacuoles appeared aberrant following AITC-exposure. However, AITC-treated seedlings of all transgenic lines tested displayed morphologies and intracellular movements similar to that of the corresponding untreated and control-treated plants, following overnight incubation in an AITC-absent environment, indicating that AITC-induced decline in actin-related movements is a reversible process. These findings provide novel insights into the cellular events in plant cells following exposure to AITC, which may further expose clues to the physiological significance of the glucosinolate-myrosinase system.http://www.mdpi.com/1422-0067/16/12/26154glucosinolatesinigrinallyl isothiocyanateactin cytoskeletonintracellular transportplant defense mechanism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bjørnar Sporsheim Anders Øverby Atle Magnar Bones |
spellingShingle |
Bjørnar Sporsheim Anders Øverby Atle Magnar Bones Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thaliana International Journal of Molecular Sciences glucosinolate sinigrin allyl isothiocyanate actin cytoskeleton intracellular transport plant defense mechanism |
author_facet |
Bjørnar Sporsheim Anders Øverby Atle Magnar Bones |
author_sort |
Bjørnar Sporsheim |
title |
Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thaliana |
title_short |
Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thaliana |
title_full |
Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thaliana |
title_fullStr |
Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thaliana |
title_full_unstemmed |
Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thaliana |
title_sort |
allyl isothiocyanate inhibits actin-dependent intracellular transport in arabidopsis thaliana |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-12-01 |
description |
Volatile allyl isothiocyanate (AITC) derives from the biodegradation of the glucosinolate sinigrin and has been associated with growth inhibition in several plants, including the model plant Arabidopsis thaliana. However, the underlying cellular mechanisms of this feature remain scarcely investigated in plants. In this study, we present evidence of an AITC-induced inhibition of actin-dependent intracellular transport in A. thaliana. A transgenic line of A. thaliana expressing yellow fluorescent protein (YFP)-tagged actin filaments was used to show attenuation of actin filament movement by AITC. This appeared gradually in a time- and dose-dependent manner and resulted in actin filaments appearing close to static. Further, we employed four transgenic lines with YFP-fusion proteins labeling the Golgi apparatus, endoplasmic reticulum (ER), vacuoles and peroxisomes to demonstrate an AITC-induced inhibition of actin-dependent intracellular transport of or, in these structures, consistent with the decline in actin filament movement. Furthermore, the morphologies of actin filaments, ER and vacuoles appeared aberrant following AITC-exposure. However, AITC-treated seedlings of all transgenic lines tested displayed morphologies and intracellular movements similar to that of the corresponding untreated and control-treated plants, following overnight incubation in an AITC-absent environment, indicating that AITC-induced decline in actin-related movements is a reversible process. These findings provide novel insights into the cellular events in plant cells following exposure to AITC, which may further expose clues to the physiological significance of the glucosinolate-myrosinase system. |
topic |
glucosinolate sinigrin allyl isothiocyanate actin cytoskeleton intracellular transport plant defense mechanism |
url |
http://www.mdpi.com/1422-0067/16/12/26154 |
work_keys_str_mv |
AT bjørnarsporsheim allylisothiocyanateinhibitsactindependentintracellulartransportinarabidopsisthaliana AT andersøverby allylisothiocyanateinhibitsactindependentintracellulartransportinarabidopsisthaliana AT atlemagnarbones allylisothiocyanateinhibitsactindependentintracellulartransportinarabidopsisthaliana |
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1725782861317931008 |