The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles

Magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, su...

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Main Authors:	Ekaterina Bobrikova, Alexey Chubarov, Elena Dmitrienko
Format:	Article
Language:	English
Published:	MDPI AG 2021-09-01
Series:	Magnetochemistry
Subjects:	iron oxide nanoparticles functionalization coating nanomedicine DNA/gene delivery systems toxicity
Online Access:	https://www.mdpi.com/2312-7481/7/9/128

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spelling	doaj-01d3631f06094d5c9799f896ad0d420f2021-09-26T00:35:13ZengMDPI AGMagnetochemistry2312-74812021-09-01712812810.3390/magnetochemistry7090128The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide NanoparticlesEkaterina Bobrikova0Alexey Chubarov1Elena Dmitrienko2Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the RAS, 630090 Novosibirsk, RussiaMagnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, such as pH, buffer components, and oligonucleotides sequences, were chosen for binding efficiency studies and oligonucleotide binding constant calculation. Standard characterization techniques, such as dynamic light scattering, zeta potential, and transmission electron microscopy, provide MNPs coating and stability. The toxicity experiments were performed using lung adenocarcinoma A549 cell line and high reactive oxygen species formation with methylene blue assay. Fe<sub>3</sub>O<sub>4</sub> MNPs complexes with oligonucleotides show high stability and excellent biocompatibility.https://www.mdpi.com/2312-7481/7/9/128iron oxide nanoparticlesfunctionalizationcoatingnanomedicineDNA/gene delivery systemstoxicity
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ekaterina Bobrikova Alexey Chubarov Elena Dmitrienko
spellingShingle	Ekaterina Bobrikova Alexey Chubarov Elena Dmitrienko The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles Magnetochemistry iron oxide nanoparticles functionalization coating nanomedicine DNA/gene delivery systems toxicity
author_facet	Ekaterina Bobrikova Alexey Chubarov Elena Dmitrienko
author_sort	Ekaterina Bobrikova
title	The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles
title_short	The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles
title_full	The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles
title_fullStr	The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles
title_full_unstemmed	The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles
title_sort	effect of ph and buffer on oligonucleotide affinity for iron oxide nanoparticles
publisher	MDPI AG
series	Magnetochemistry
issn	2312-7481
publishDate	2021-09-01
description	Magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, such as pH, buffer components, and oligonucleotides sequences, were chosen for binding efficiency studies and oligonucleotide binding constant calculation. Standard characterization techniques, such as dynamic light scattering, zeta potential, and transmission electron microscopy, provide MNPs coating and stability. The toxicity experiments were performed using lung adenocarcinoma A549 cell line and high reactive oxygen species formation with methylene blue assay. Fe<sub>3</sub>O<sub>4</sub> MNPs complexes with oligonucleotides show high stability and excellent biocompatibility.
topic	iron oxide nanoparticles functionalization coating nanomedicine DNA/gene delivery systems toxicity
url	https://www.mdpi.com/2312-7481/7/9/128
work_keys_str_mv	AT ekaterinabobrikova theeffectofphandbufferonoligonucleotideaffinityforironoxidenanoparticles AT alexeychubarov theeffectofphandbufferonoligonucleotideaffinityforironoxidenanoparticles AT elenadmitrienko theeffectofphandbufferonoligonucleotideaffinityforironoxidenanoparticles AT ekaterinabobrikova effectofphandbufferonoligonucleotideaffinityforironoxidenanoparticles AT alexeychubarov effectofphandbufferonoligonucleotideaffinityforironoxidenanoparticles AT elenadmitrienko effectofphandbufferonoligonucleotideaffinityforironoxidenanoparticles
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The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles

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