Proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: Effects of topical treatment
Cerebral palsy (CP) is a non-progressive motor-impairment disorder related to brain injury early in development. To gain new insights into the mechanisms of CP and the therapeutic efficacy of Baimai ointment, we used a high-throughput quantitative proteomic approach to evaluate proteomic changes in...
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doaj-01d930571af444d1b7e565059ef593a12021-05-21T04:18:26ZengElsevierBiomedicine & Pharmacotherapy0753-33222021-01-01133110844Proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: Effects of topical treatmentTao Wang0Yusheng Zhang1Weiwu Chen2Jin Tao3Qiao Xue4Wei Ge5Wanchen Dou6Chao Ma7Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Human Anatomy, Histology and Embryology, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Dongcheng District, Beijing 100005, ChinaNational Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Dongcheng District, Beijing 100005, ChinaSchool of Business Administration, Shenyang Pharmaceutical University, Shenyang, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Human Anatomy, Histology and Embryology, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Dongcheng District, Beijing 100005, ChinaTibet Cheezheng Tibetan Medicine Co., Ltd., Beijing, ChinaNational Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Dongcheng District, Beijing 100005, ChinaDepartment of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China; Corresponding authors.Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Human Anatomy, Histology and Embryology, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Dongcheng District, Beijing 100005, China; Corresponding authors.Cerebral palsy (CP) is a non-progressive motor-impairment disorder related to brain injury early in development. To gain new insights into the mechanisms of CP and the therapeutic efficacy of Baimai ointment, we used a high-throughput quantitative proteomic approach to evaluate proteomic changes in the hippocampus and motor cortex in a rat model of CP induced by lipopolysaccharide (LPS) combined with hypoxia/ischemia (H/I). More than 2000 proteins were identified in each brain region with high confidence. Quantitative analysis demonstrated profound disturbances in the proteomes of the hippocampus and motor cortex after LPS + H/I, in addition to the disruption of the motor system. In contrast, the topical application of Baimai ointment not only alleviated the motor deficit in the CP model rats, but also restored the proteomes in the brain cortex. Furthermore, astrocytes in the hippocampus were strongly activated in the Baimai-treated CP rat brains, associated with an increase in neurotrophic factors. Proteomic analysis demonstrated that the CP model induced neuroinflammatory responses in the brain which were reversed by the topical application of Baimai ointment. This study highlights the unexpected roles of hippocampus and motor cortex neurons in CP progress and treatment, thus providing potentially novel therapeutic targets for CP.http://www.sciencedirect.com/science/article/pii/S0753332220310362HippocampusMotor cortexCerebral palsyProteomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tao Wang Yusheng Zhang Weiwu Chen Jin Tao Qiao Xue Wei Ge Wanchen Dou Chao Ma |
spellingShingle |
Tao Wang Yusheng Zhang Weiwu Chen Jin Tao Qiao Xue Wei Ge Wanchen Dou Chao Ma Proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: Effects of topical treatment Biomedicine & Pharmacotherapy Hippocampus Motor cortex Cerebral palsy Proteomics |
author_facet |
Tao Wang Yusheng Zhang Weiwu Chen Jin Tao Qiao Xue Wei Ge Wanchen Dou Chao Ma |
author_sort |
Tao Wang |
title |
Proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: Effects of topical treatment |
title_short |
Proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: Effects of topical treatment |
title_full |
Proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: Effects of topical treatment |
title_fullStr |
Proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: Effects of topical treatment |
title_full_unstemmed |
Proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: Effects of topical treatment |
title_sort |
proteomic changes in the hippocampus and motor cortex in a rat model of cerebral palsy: effects of topical treatment |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2021-01-01 |
description |
Cerebral palsy (CP) is a non-progressive motor-impairment disorder related to brain injury early in development. To gain new insights into the mechanisms of CP and the therapeutic efficacy of Baimai ointment, we used a high-throughput quantitative proteomic approach to evaluate proteomic changes in the hippocampus and motor cortex in a rat model of CP induced by lipopolysaccharide (LPS) combined with hypoxia/ischemia (H/I). More than 2000 proteins were identified in each brain region with high confidence. Quantitative analysis demonstrated profound disturbances in the proteomes of the hippocampus and motor cortex after LPS + H/I, in addition to the disruption of the motor system. In contrast, the topical application of Baimai ointment not only alleviated the motor deficit in the CP model rats, but also restored the proteomes in the brain cortex. Furthermore, astrocytes in the hippocampus were strongly activated in the Baimai-treated CP rat brains, associated with an increase in neurotrophic factors. Proteomic analysis demonstrated that the CP model induced neuroinflammatory responses in the brain which were reversed by the topical application of Baimai ointment. This study highlights the unexpected roles of hippocampus and motor cortex neurons in CP progress and treatment, thus providing potentially novel therapeutic targets for CP. |
topic |
Hippocampus Motor cortex Cerebral palsy Proteomics |
url |
http://www.sciencedirect.com/science/article/pii/S0753332220310362 |
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