The citotoxicity of calcium hydroxide intracanal dressing by MTT assay

• Main Author: Nanik Zubaidah
• Format: Article
• Language: English
• Published: Universitas Airlangga 2007-12-01
• Series: Dental Journal: Majalah Kedokteran Gigi
• Subjects: calcium hydroxide; citotoxicity; MTT assay
• Online Access: http://e-journal.unair.ac.id/index.php/MKG/article/view/1033
• ISSN: 1978-3728; 2442-9740

<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Calcium hydroxide had been used as the intracanal dressing in endodontic treatment due to its high alkaline and high antimicrobial </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>capacity. It also be able to dissolve the necrotic tissue, prevent the root resorbtion and regenerate a new hard tissue. The aim of this </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>study is to identify the concentration of calcium hydroxide that has the lowest citotoxicity. There are 5 groups, each group had 8 </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>samples with different concentration of calcium hydroxide. Group I: 50%, Group II: 55%, Group III: 60%, Group IV: 65% and Group </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>V: 70%. The citotoxicity test by using enzymatic assay of MTT [3-(4.5- dimethylthiazol-2yl) ]-2.5 diphenyl tetrazolium bromide, against </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>fibroblast cell (BHK-21). The result of susceptibility test was showed by the citotoxicity detection of the survive cell of fibroblast that was </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>measured spectrophotometrically using 595 nm beam. The data was analyzed using One-Way ANOVA test with significant difference </em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">α = <span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>0.05 and subsequently LSD test. The result showed that in concentration 50%, 55%, 60%, 65%, and 70% calcium hydroxide had </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>low toxicity, but calcium hydroxide 60%, had the lowest toxicity.</em></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span>