Landscape of Genome-Wide DNA Methylation of Colorectal Cancer Metastasis
Colorectal cancer is a heterogeneous disease caused by both genetic and epigenetics factors. Analysing DNA methylation changes occurring during colorectal cancer progression and metastasis formation is crucial for the identification of novel epigenetic markers of patient prognosis. Genome-wide methy...
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doaj-01e500b134f94a4896b4d7fe670550742020-11-25T03:42:50ZengMDPI AGCancers2072-66942020-09-01122710271010.3390/cancers12092710Landscape of Genome-Wide DNA Methylation of Colorectal Cancer MetastasisCarmen Ili0Kurt Buchegger1Hannah Demond2Juan Castillo-Fernandez3Gavin Kelsey4Louise Zanella5Michel Abanto6Ismael Riquelme7Jaime López8Tamara Viscarra9Patricia García10Enrique Bellolio11David Saavedra12Priscilla Brebi13Laboratory of Integrative Biology (LIBi), Centro de Excelencia en Medicina Traslacional (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4810296, ChileLaboratory of Integrative Biology (LIBi), Centro de Excelencia en Medicina Traslacional (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4810296, ChileLaboratory of Integrative Biology (LIBi), Centro de Excelencia en Medicina Traslacional (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4810296, ChileEpigenetics Programme, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UKEpigenetics Programme, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UKLaboratory of Integrative Biology (LIBi), Centro de Excelencia en Medicina Traslacional (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4810296, ChileScientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811230, ChileInstituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Temuco 4810101, ChileLaboratory of Integrative Biology (LIBi), Centro de Excelencia en Medicina Traslacional (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4810296, ChileLaboratory of Integrative Biology (LIBi), Centro de Excelencia en Medicina Traslacional (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4810296, ChileDepartment of Pathology, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago 8330034, ChileDepartamento Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco 4781180, ChileDepartamento de Medicina Interna, Hospital Hernán Henríquez Aravena, Temuco 4781151, ChileLaboratory of Integrative Biology (LIBi), Centro de Excelencia en Medicina Traslacional (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4810296, ChileColorectal cancer is a heterogeneous disease caused by both genetic and epigenetics factors. Analysing DNA methylation changes occurring during colorectal cancer progression and metastasis formation is crucial for the identification of novel epigenetic markers of patient prognosis. Genome-wide methylation sequencing of paired samples of colon (normal adjacent, primary tumour and lymph node metastasis) showed global hypomethylation and CpG island (CGI) hypermethylation of primary tumours compared to normal. In metastasis we observed high global and non-CGI regions methylation, but lower CGI methylation, compared to primary tumours. Gene ontology analysis showed shared biological processes between hypermethylated CGIs in metastasis and primary tumours. After complementary analysis with The Cancer Genome Atlas (TCGA) cohort, <i>FIGN</i>, <i>HTRA3</i>, <i>BDNF</i>, <i>HCN4</i> and <i>STAC2</i> genes were found associated with poor survival. We mapped the methylation landscape of colon normal tissues, primary tumours and lymph node metastasis, being capable of identified methylation changes throughout the genome. Furthermore, we found five genes with potential for methylation biomarkers of poor prognosis in colorectal cancer patients.https://www.mdpi.com/2072-6694/12/9/2710colorectal cancermetastasisDNA methylationlymph nodegenome-wide analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carmen Ili Kurt Buchegger Hannah Demond Juan Castillo-Fernandez Gavin Kelsey Louise Zanella Michel Abanto Ismael Riquelme Jaime López Tamara Viscarra Patricia García Enrique Bellolio David Saavedra Priscilla Brebi |
spellingShingle |
Carmen Ili Kurt Buchegger Hannah Demond Juan Castillo-Fernandez Gavin Kelsey Louise Zanella Michel Abanto Ismael Riquelme Jaime López Tamara Viscarra Patricia García Enrique Bellolio David Saavedra Priscilla Brebi Landscape of Genome-Wide DNA Methylation of Colorectal Cancer Metastasis Cancers colorectal cancer metastasis DNA methylation lymph node genome-wide analysis |
author_facet |
Carmen Ili Kurt Buchegger Hannah Demond Juan Castillo-Fernandez Gavin Kelsey Louise Zanella Michel Abanto Ismael Riquelme Jaime López Tamara Viscarra Patricia García Enrique Bellolio David Saavedra Priscilla Brebi |
author_sort |
Carmen Ili |
title |
Landscape of Genome-Wide DNA Methylation of Colorectal Cancer Metastasis |
title_short |
Landscape of Genome-Wide DNA Methylation of Colorectal Cancer Metastasis |
title_full |
Landscape of Genome-Wide DNA Methylation of Colorectal Cancer Metastasis |
title_fullStr |
Landscape of Genome-Wide DNA Methylation of Colorectal Cancer Metastasis |
title_full_unstemmed |
Landscape of Genome-Wide DNA Methylation of Colorectal Cancer Metastasis |
title_sort |
landscape of genome-wide dna methylation of colorectal cancer metastasis |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2020-09-01 |
description |
Colorectal cancer is a heterogeneous disease caused by both genetic and epigenetics factors. Analysing DNA methylation changes occurring during colorectal cancer progression and metastasis formation is crucial for the identification of novel epigenetic markers of patient prognosis. Genome-wide methylation sequencing of paired samples of colon (normal adjacent, primary tumour and lymph node metastasis) showed global hypomethylation and CpG island (CGI) hypermethylation of primary tumours compared to normal. In metastasis we observed high global and non-CGI regions methylation, but lower CGI methylation, compared to primary tumours. Gene ontology analysis showed shared biological processes between hypermethylated CGIs in metastasis and primary tumours. After complementary analysis with The Cancer Genome Atlas (TCGA) cohort, <i>FIGN</i>, <i>HTRA3</i>, <i>BDNF</i>, <i>HCN4</i> and <i>STAC2</i> genes were found associated with poor survival. We mapped the methylation landscape of colon normal tissues, primary tumours and lymph node metastasis, being capable of identified methylation changes throughout the genome. Furthermore, we found five genes with potential for methylation biomarkers of poor prognosis in colorectal cancer patients. |
topic |
colorectal cancer metastasis DNA methylation lymph node genome-wide analysis |
url |
https://www.mdpi.com/2072-6694/12/9/2710 |
work_keys_str_mv |
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