Does Citrulline Have Protective Effects on Liver Injury in Septic Rats?
Citrulline (Cit) supplementation was proposed to serve as a therapeutic intervention to restore arginine (Arg) concentrations and improve related functions in sepsis. This study explored whether citrulline had positive effects on liver injury and cytokine release in the early stages of sepsis. The c...
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doaj-01e915640cab4a4499587da3b3326c352020-11-25T00:02:46ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/14695901469590Does Citrulline Have Protective Effects on Liver Injury in Septic Rats?Bin Cai0Yu-long Luo1Shi-jun Wang2Wei-yuan Wei3Xue-hui Zhang4Wei Huang5Tong Li6Meng Zhang7Nan Wu8Gopaul Roodrajeetsing9Sen Zhang10Department of Colorectal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Colorectal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Colorectal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Gastrointestinal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Nuclear Medicine, People’s Hospital of Beihai City, Beihai, Guangxi 536000, ChinaDepartment of Colorectal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Colorectal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Colorectal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Colorectal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Neurosurgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Colorectal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaCitrulline (Cit) supplementation was proposed to serve as a therapeutic intervention to restore arginine (Arg) concentrations and improve related functions in sepsis. This study explored whether citrulline had positive effects on liver injury and cytokine release in the early stages of sepsis. The cecal ligation and puncture (CLP) model was utilized in our study. Rats were divided into four groups: normal, Cit, CLP, and CLP+Cit. The CLP group and CLP+Cit group were separated into 6-, 12-, and 24-hour groups, according to the time points of sacrifice after surgery. Intragastric administration of L-citrulline was applied to rats in Cit and CLP+Cit groups before surgery. Serum AST and ALT levels and levels of MDA, SOD, NO, and iNOS in the liver tissues were evaluated. Plasma concentrations of Cit and Arg were assessed using HPLC-MS/MS. Serum concentrations of cytokines and chemokines were calculated by Luminex. Results showed SOD activities of CLP+Cit groups were significantly higher than that of CLP groups, contrasting with the MDA and NO levels which were significantly lower in CLP+Cit groups than in CLP groups. In addition, plasma concentrations of TNF-α, IL-6, and IL-1β were significantly lower in the CLP+Cit 6-hour group than in the CLP 6-hour group.http://dx.doi.org/10.1155/2016/1469590 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bin Cai Yu-long Luo Shi-jun Wang Wei-yuan Wei Xue-hui Zhang Wei Huang Tong Li Meng Zhang Nan Wu Gopaul Roodrajeetsing Sen Zhang |
spellingShingle |
Bin Cai Yu-long Luo Shi-jun Wang Wei-yuan Wei Xue-hui Zhang Wei Huang Tong Li Meng Zhang Nan Wu Gopaul Roodrajeetsing Sen Zhang Does Citrulline Have Protective Effects on Liver Injury in Septic Rats? BioMed Research International |
author_facet |
Bin Cai Yu-long Luo Shi-jun Wang Wei-yuan Wei Xue-hui Zhang Wei Huang Tong Li Meng Zhang Nan Wu Gopaul Roodrajeetsing Sen Zhang |
author_sort |
Bin Cai |
title |
Does Citrulline Have Protective Effects on Liver Injury in Septic Rats? |
title_short |
Does Citrulline Have Protective Effects on Liver Injury in Septic Rats? |
title_full |
Does Citrulline Have Protective Effects on Liver Injury in Septic Rats? |
title_fullStr |
Does Citrulline Have Protective Effects on Liver Injury in Septic Rats? |
title_full_unstemmed |
Does Citrulline Have Protective Effects on Liver Injury in Septic Rats? |
title_sort |
does citrulline have protective effects on liver injury in septic rats? |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2016-01-01 |
description |
Citrulline (Cit) supplementation was proposed to serve as a therapeutic intervention to restore arginine (Arg) concentrations and improve related functions in sepsis. This study explored whether citrulline had positive effects on liver injury and cytokine release in the early stages of sepsis. The cecal ligation and puncture (CLP) model was utilized in our study. Rats were divided into four groups: normal, Cit, CLP, and CLP+Cit. The CLP group and CLP+Cit group were separated into 6-, 12-, and 24-hour groups, according to the time points of sacrifice after surgery. Intragastric administration of L-citrulline was applied to rats in Cit and CLP+Cit groups before surgery. Serum AST and ALT levels and levels of MDA, SOD, NO, and iNOS in the liver tissues were evaluated. Plasma concentrations of Cit and Arg were assessed using HPLC-MS/MS. Serum concentrations of cytokines and chemokines were calculated by Luminex. Results showed SOD activities of CLP+Cit groups were significantly higher than that of CLP groups, contrasting with the MDA and NO levels which were significantly lower in CLP+Cit groups than in CLP groups. In addition, plasma concentrations of TNF-α, IL-6, and IL-1β were significantly lower in the CLP+Cit 6-hour group than in the CLP 6-hour group. |
url |
http://dx.doi.org/10.1155/2016/1469590 |
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