Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation
Previously, we established a microRNA (miRNA) expression signature in pancreatic ductal adenocarcinoma (PDAC) tissues using RNA sequencing and found significantly reduced expression of miR-204-5p. Here, we aimed to investigate the functional significance of miR-204-5p and to identify miR-204-5p targ...
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doaj-01eb3424f464454682d0af2e206377c72020-11-24T22:07:53ZengMDPI AGCancers2072-66942019-03-0111332710.3390/cancers11030327cancers11030327Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 RegulationMuhammad Khalid0Tetsuya Idichi1Naohiko Seki2Masumi Wada3Yasutaka Yamada4Haruhi Fukuhisa5Hiroko Toda6Yoshiaki Kita7Yota Kawasaki8Kiyonori Tanoue9Hiroshi Kurahara10Yuko Mataki11Kosei Maemura12Shoji Natsugoe13Department of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Functional Genomics, Chiba University Graduate School of Medicine, Chiba 260-8670, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Functional Genomics, Chiba University Graduate School of Medicine, Chiba 260-8670, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanDepartment of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima 890-8580, JapanPreviously, we established a microRNA (miRNA) expression signature in pancreatic ductal adenocarcinoma (PDAC) tissues using RNA sequencing and found significantly reduced expression of miR-204-5p. Here, we aimed to investigate the functional significance of miR-204-5p and to identify miR-204-5p target genes involved in PDAC pathogenesis. Cancer cell migration and invasion were significantly inhibited by ectopic expression of miR-204-5p in PDAC cells. Comprehensive gene expression analyses and in silico database searches revealed 25 putative targets regulated by miR-204-5p in PDAC cells. Among these target genes, high expression levels of RACGAP1, DHRS9, AP1S3, FOXC1, PRP11, RHBDL2 and MUC4 were significant predictors of a poor prognosis of patients with PDAC. In this study, we focused on RACGAP1 (Rac guanosine triphosphatase-activating protein 1) because its expression was most significantly predictive of PDAC pathogenesis (overall survival rate: p = 0.0000548; disease-free survival rate: p = 0.0014). Overexpression of RACGAP1 was detected in PDAC clinical specimens, and its expression enhanced the migration and invasion of PDAC cells. Moreover, downstream genes affected by RACGAP1 (e.g., MMP28, CEP55, CDK1, ANLN and S100A14) are involved in PDAC pathogenesis. Our strategy to identify antitumor miRNAs and their target genes will help elucidate the molecular pathogenesis of PDAC.http://www.mdpi.com/2072-6694/11/3/327microRNAmiR-204-5pantitumorpancreatic ductal adenocarcinomapathogenesisRACGAP1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Khalid Tetsuya Idichi Naohiko Seki Masumi Wada Yasutaka Yamada Haruhi Fukuhisa Hiroko Toda Yoshiaki Kita Yota Kawasaki Kiyonori Tanoue Hiroshi Kurahara Yuko Mataki Kosei Maemura Shoji Natsugoe |
spellingShingle |
Muhammad Khalid Tetsuya Idichi Naohiko Seki Masumi Wada Yasutaka Yamada Haruhi Fukuhisa Hiroko Toda Yoshiaki Kita Yota Kawasaki Kiyonori Tanoue Hiroshi Kurahara Yuko Mataki Kosei Maemura Shoji Natsugoe Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation Cancers microRNA miR-204-5p antitumor pancreatic ductal adenocarcinoma pathogenesis RACGAP1 |
author_facet |
Muhammad Khalid Tetsuya Idichi Naohiko Seki Masumi Wada Yasutaka Yamada Haruhi Fukuhisa Hiroko Toda Yoshiaki Kita Yota Kawasaki Kiyonori Tanoue Hiroshi Kurahara Yuko Mataki Kosei Maemura Shoji Natsugoe |
author_sort |
Muhammad Khalid |
title |
Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation |
title_short |
Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation |
title_full |
Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation |
title_fullStr |
Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation |
title_full_unstemmed |
Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation |
title_sort |
gene regulation by antitumor mir-204-5p in pancreatic ductal adenocarcinoma: the clinical significance of direct racgap1 regulation |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2019-03-01 |
description |
Previously, we established a microRNA (miRNA) expression signature in pancreatic ductal adenocarcinoma (PDAC) tissues using RNA sequencing and found significantly reduced expression of miR-204-5p. Here, we aimed to investigate the functional significance of miR-204-5p and to identify miR-204-5p target genes involved in PDAC pathogenesis. Cancer cell migration and invasion were significantly inhibited by ectopic expression of miR-204-5p in PDAC cells. Comprehensive gene expression analyses and in silico database searches revealed 25 putative targets regulated by miR-204-5p in PDAC cells. Among these target genes, high expression levels of RACGAP1, DHRS9, AP1S3, FOXC1, PRP11, RHBDL2 and MUC4 were significant predictors of a poor prognosis of patients with PDAC. In this study, we focused on RACGAP1 (Rac guanosine triphosphatase-activating protein 1) because its expression was most significantly predictive of PDAC pathogenesis (overall survival rate: p = 0.0000548; disease-free survival rate: p = 0.0014). Overexpression of RACGAP1 was detected in PDAC clinical specimens, and its expression enhanced the migration and invasion of PDAC cells. Moreover, downstream genes affected by RACGAP1 (e.g., MMP28, CEP55, CDK1, ANLN and S100A14) are involved in PDAC pathogenesis. Our strategy to identify antitumor miRNAs and their target genes will help elucidate the molecular pathogenesis of PDAC. |
topic |
microRNA miR-204-5p antitumor pancreatic ductal adenocarcinoma pathogenesis RACGAP1 |
url |
http://www.mdpi.com/2072-6694/11/3/327 |
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